Method for determining the application of a sample fluid on an analyte strip using first and second derivatives

• US 6,069,011 A
• Filed: 12/10/1997
• Issued: 05/30/2000
• Est. Priority Date: 12/10/1997
• Status: Expired due to Fees

• Current Assignee: UMM Electronics, Inc. (Nortech Systems, Inc.)
• Original Assignee: UMM Electronics, Inc. (Nortech Systems, Inc.)
• Inventors: Riedel, Richard A.
• Primary Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US08/988,304
• Time in Patent Office: 902 Days
• Field of Search: 436/34, 436/63, 436/95, 436/164, 436/166, 436/169, 422/56, 422/58, 422/68.1, 422/82.05, 422/82.02, 422/82.01, 435/4, 435/14, 435/25, 435/28, 435/805, 435/808
• US Class Current: 436/34
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

A method for determining the application of a sample fluid on an analyte strip using first and second derivatives. Sample fluid, such as whole blood, is applied to a reagent matrix and sensor readings, such as reflectance readings or current readings, are taken of the fluid/reagent combination. The first and second derivatives of the sensor readings are calculated. A predetermined incubation time period is begun at either a local minimum in the first derivative or a local maximum following a local minimum in the second derivative data. At the expiration of the incubation period, another sensor reading is taken which is indicative of an analyte concentration in the sample fluid (such as glucose concentration in a whole blood sample).

Claims

1. A method for determining a start of reaction timing for measurement of a reaction between a sample fluid and a reagent, comprising the steps of:

a) measuring light reflected from the reagent at intervals prior to application of the sample fluid to the reagent;

b) applying the sample fluid to the reagent, thereby creating a reagent/sample fluid combination;

c) measuring light reflected from the reagent/sample fluid combination at intervals after performing step (b);

d) calculating a first derivative of measurement data taken at steps (a) and (c);

e) determining a location of a local minimum in the first derivative;

f) beginning a predetermined time period at a time corresponding to the local minimum, wherein the predetermined time period corresponds to a time for reaction between the sample fluid and reagent; and

g) measuring light reflected from the reagent/sample fluid combination after expiration of the predetermined time period.

2. The method of claim 1, wherein the sample fluid is whole blood.

3. The method of claim 2, wherein the reagent reacts with the whole blood to produce a color change in proportion to an amount of glucose in the whole blood.

4. The method of claim 1, wherein the intervals are fixed intervals.

5. A method for determining a start of reaction timing for measurement of a reaction between a sample fluid and a reagent, comprising the steps of:

a) measuring light reflected from the reagent at intervals prior to application of the sample fluid to the reagent;

b) applying the sample fluid to the reagent, thereby creating a reagent/sample fluid combination;

c) measuring light reflected from the reagent/sample fluid combination at intervals after performing step (b);

d) calculating a second derivative of measurement data taken at steps (a) and (c);

e) determining a location of a local maximum following a local minimum in the second derivative;

f) beginning a predetermined time period at a time corresponding to the local maximum, wherein the predetermined time period corresponds to a time for reaction between the sample fluid and reagent; and

g) measuring light reflected from the reagent/sample fluid combination after expiration of the predetermined time period.

6. The method of claim 5, wherein the sample fluid is whole blood.

7. The method of claim 6, wherein the reagent reacts with the whole blood to produce a color change in proportion to an amount of glucose in the whole blood.

8. The method of claim 5, wherein the intervals are fixed intervals.

9. A method for determining a start of reaction timing for measurement of a reaction between a sample fluid and a reagent, comprising the steps of:

a) measuring current flowing through the reagent at intervals prior to application of the sample fluid to the reagent;

b) applying the sample fluid to the reagent, thereby creating a reagent/sample fluid combination;

c) measuring current flowing through the reagent/sample fluid combination at intervals after performing step (b);

d) calculating a first derivative of measurement data taken at steps (a) and (c);

e) determining a location of a local minimum in the first derivative;

f) beginning a predetermined time period at a time corresponding to the local minimum, wherein the predetermined time period corresponds to a time for reaction between the sample fluid and reagent; and

g) measuring current flowing through the reagent/sample fluid combination after expiration of the predetermined time period.

10. The method of claim 9, wherein the sample fluid is whole blood.

11. The method of claim 10, wherein the reagent reacts with the whole blood to produce a color change in proportion to an amount of glucose in the whole blood.

12. The method of claim 9, wherein the intervals are fixed intervals.

13. A method for determining a start of reaction timing for measurement of a reaction between a sample fluid and a reagent, comprising the steps of:

a) measuring current flowing through the reagent at intervals prior to application of the sample fluid to the reagent;

b) applying the sample fluid to the reagent, thereby creating a reagent/sample fluid combination;

c) measuring current flowing through the reagent/sample fluid combination at intervals after performing step (b);

d) calculating a second derivative of measurement data taken at steps (a) and (c);

e) determining a location of a local maximum following a local minimum in the second derivative;

f) beginning a predetermined time period at a time corresponding to the local maximum, wherein the predetermined time period corresponds to a time for reaction between the sample fluid and reagent; and

g) measuring current flowing through the reagent/sample fluid combination after expiration of the predetermined time period.

14. The method of claim 13, wherein the sample fluid is whole blood.

15. The method of claim 14, wherein the reagent reacts with the whole blood to produce a color change proportion to an amount of glucose in the whole blood.

16. The method of claim 13, wherein the intervals are fixed intervals.