Optical imaging of tissue using inelastically scattered light

• US 6,070,583 A
• Filed: 02/21/1995
• Issued: 06/06/2000
• Est. Priority Date: 02/21/1995
• Status: Expired due to Fees

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Dasari, Ramachandra Roa, Wang, Yang, Wu, Jun, Perelman, Lev T., Feld, Michael S., Itzkan, Irving
• Primary Examiner(s): Lateef, Marvin M.
• Assistant Examiner(s): MANTIS MERCADER, ELENI M
• Application Number:
  US08/391,209
• Time in Patent Office: 1,932 Days
• Field of Search: 128/664, 128/665, 128/633, 128/634, 356/433, 356/435, 356/342, 356/343, 356/338, 356/432, 250/330, 250/331, 250/332, 250/333, 250/334, 250/574, 250/575, 250/358.1
• US Class Current: 600/476
• CPC Class Codes: A61B 5/0073 by tomography, i.e. reconstruction of 3D images from 2D projections A61B5/0066 takes precedence
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B

Abstract

The present invention relates to the use of inelastically scattered light, particularly fluorescent and Raman scattered light, for determining the location and composition of material within various organs of the human body. The systems and methods of the present invention provide for medical imaging in three dimensions as well as histological information that can be used for diagnosing disease and various medical conditions.

Claims

1. A method for determining a location of material within tissue comprising;

irradiating tissue with radiation, the tissue having material located underneath a surface of the tissue;

collecting inelastically scattered radiation from the material at a plurality of at least three locations on different sides of the tissue and defining a plane extending through the tissue; and

determining the location of the material within the tissue.

2. The method of claim 1 further comprising providing a detector that detects the collected radiation and generating a three dimensional image of the material from the detected radiation from the material, the inelastically scattered radiation comprising fluorescent radiation.

3. The method of claim 1 further comprising detecting Raman scattered radiation from the material.

4. The method of claim 1 further comprising providing a plurality of optical collectors positioned around the tissue to collect light from the tissue.

5. The method of claim 1 further comprising providing a plurality of optical fiber, each having a distal end and a proximal end, the distal ends of the fibers being positioned about the tissue to collect light from the tissue.

6. The method of claim 1 further comprising detecting the collected radiation with a streak camera.

7. The method of claim 1 wherein the determining step further comprises measuring an increase in inelastically scattered radiation from the tissue as a function of time.

8. A method for determining a location of material within tissue comprising;

providing a laser that emits light at an excitation wavelength;

providing a plurality of optical collectors around the tissue and a detector, the collectors being optically coupled to the detector;

irradiating tissue with radiation at the excitation wavelength, the tissue having a lesion located underneath a surface of the tissue;

collecting inelastically scattered radiation from the lesion at least at three locations on different sides of the tissue, the three locations defining a plane extending through the tissue; and

detecting with the detector the collected light;

generating a three dimensional representation of the lesion with the detected light; and

determining the location of the lesion within the tissue.

9. The method of claim 8 wherein the detector detects the collected radiation and generating a three dimensional image of the lesion from the detected fluorescent radiation from the material the inelastically scattered radiation comprising fluorescent radiation.

10. The method of claim 8 further comprising detecting Raman scattered radiation from the material.

11. The method of claim 8 further comprising providing a plurality of optical fibers positioned around the tissue to collect light from the lesion.

12. The method of claim 8 further comprising determining the presence of a fluorophore in the lesion.

13. The method of claim 8 further comprising determining a concentration of an analyte in the lesion.

14. The method of claim 8 further comprising measuring an intensity of radiation from the lesion as a function of time from the emission of the excitation wavelength.

15. The method of claim 8 further comprising:

collecting fluorescent light from the tissue at least at three angles;

detecting and comparing the time-dependent fluorescent light at each of the three angles; and

forming an image of fluorescing tissue from the compared fluorescent signals and determining a depth of the tissue within the medium.

16. A tomographic system for determining a location of material within tissue comprising;

a light source that emits an excitation wavelength of radiation, the light source being optically coupled to irradiate tissue with radiation, the tissue having material located underneath a surface of the tissue;

an optical collector that collects inelastically scattered radiation from the material at least at three locations on different sides of the tissue, the three locations defining a plane extending through the tissue;

a detector optically coupled to the optical collector; and

a data processor that determines the location of the material within the tissue.

17. The system of claim 16 further comprising an image processor that generates a three dimensional image of the material from the detected radiation from the material, the inelastically scattered radiation comprising fluroescent radiation.

18. The system of claim 16 further comprising a filter for selectively transmitting Raman scattered radiation from the material.

19. The system of claim 16 wherein the optical collector comprises a plurality of optical fibers positioned around the tissue.

20. The system of claim 16 wherein the optical collector comprises a plurality of collectors that are positioned concentrically about the tissue.