Boron-containing hormone analogs and methods of their use in imaging or killing cells having hormone receptors

• US 6,074,625 A
• Filed: 02/28/1997
• Issued: 06/13/2000
• Est. Priority Date: 06/27/1994
• Status: Expired due to Fees

• Current Assignee: Neutron Therapeutics Inc.
• Original Assignee: Neutron Therapeutics Inc.
• Inventors: Groudine, Mark T., Hawthorne, M. Frederick
• Primary Examiner(s): Dees, Jose' G.
• Assistant Examiner(s): JONES, DAMERON LEVEST
• Application Number:
  US08/808,203
• Time in Patent Office: 1,201 Days
• Field of Search: 424/1.11, 424/1.33, 424/1.37, 424/1.61, 424/1.81, 424/9.1, 424/1.45, 424/1.65
• US Class Current: 424/1.11
• CPC Class Codes: A61K 41/0095 Boron neutron capture therapy, i.e. BNCT, e.g. using boronated porphyrins

Abstract

Boron containing compounds are targeted to hormonally responsive cells by contacting the cells with a conjugate of a boron containing moiety conjugated to a ligand having binding specificity for an intracellular receptor of the cell. The conjugates are useful for imaging or killing hormonally responsive cells, such as hormonally responsive tumor cells. For target cell killing, the cells are contacted with a ¹⁰ B containing moiety conjugated to a ligand having binding specificity for an intracellular hormone receptor of the cells. The ¹⁰ B containing moiety becomes associated with the hormone receptor of the cells, which may then be irradiated with neutrons to kill the cells by boron neutron capture. For target cell imaging, the cells are contacted with a ¹¹ B containing moiety conjugated to a ligand having binding specificity for an intracellular hormone receptor of the cells, and the boron that becomes associated with the hormone receptor of the cells may then be imaged using magnetic resonance imaging techniques.

Claims

1. A method of targeting boron to cells that contain intracellular receptors comprising contacting the cells with a conjugate comprising a boron containing moiety, wherein the boron containing moiety contains at least nine ¹⁰ B atoms, conjugated to a ligand having binding specificity for it least one intracellular receptor of the cells.

3. The method of claim 1 wherein the cells are tumor cells.

2. A method of killing intracellular receptor containing cells comprising contacting the cells with a boron neutron capture agent comprising a ¹⁰ B containing moiety, wherein the ¹⁰ B containing moiety contains at least nine ¹⁰ B atoms, conjugated to a ligand having binding specificity for at least one intracellular receptor of the cells, and then irradiating the cells with neutrons.

4. The method of claim 2 wherein the cells are metastatic breast cancer cells and the ligand comprises a ¹⁰ B containing moiety conjugated to ligand having binding specificity for estrogen receptors.

5. The method of claim 4 wherein the ligand comprises a ¹⁰ B containing moiety conjugated to tamoxifen.

6. The method of claim 2 wherein the cells are prostate cancer cells and the ligand comprises a ¹⁰ B containing moiety conjugated to ligand having binding specificity for androgen receptors.

7. The method of claim 6 wherein the ligand comprises a ¹⁰ B containing moiety conjugated to a 4'"'"'-substituted or a 3'"'"',4'"'"'-disubstituted anilide residue.

8. The method of claim 7 wherein the anilide residue is 4'"'"'-nitro-3'"'"'-trifluoromethyl-isobutyranilide residue.

9. The method of claim 6 wherein the ligand comprises a ¹⁰ B containing moiety conjugated to a cyproterone acetate residue.

10. The method of claim 2 wherein the cells are leukemia cells and the ligand comprises a ¹⁰ B containing moiety conjugated to ligand having binding specificity for glucocorticoid receptors.

11. The method of claim 2 wherein the cells are renal carcinoma cells and the ligand comprises a ¹⁰ B containing moiety conjugated to ligand having binding specificity for progesterone receptors, androgen receptors or glucocorticoid receptors.

12. The method of claim 2 wherein the cells are endometrial cancer cells and the ligand comprises a ¹⁰ B containing moiety conjugated to ligand having binding specificity for estrogen receptors or progesterone receptors.

13. The method of claim 2 wherein the cells are ovarian carcinoma cells and the ligand comprises a ¹⁰ B containing moiety conjugated to ligand having binding specificity for estrogen receptors or progesterone receptors.

14. The method of claim 2 wherein the cells are carcinoma cells of the cervix, vagina or vulva and the ligand comprises a ¹⁰ B containing moiety conjugated to ligand having binding specificity for estrogen receptors or progesterone receptors.

15. The method of claim 2 wherein the ¹⁰ B containing moiety comprises at least one carborane residue.

16. The method of claim 2 wherein the cells are irradiated with thermal neutrons.

17. The method of claim 2 wherein the cells are irradiated with epithermal neutrons.

18. The method of claim 2 wherein the cells are irradiated with fast neutrons.

19. A method of killing intracellular receptor containing cells comprising contacting the cells with a boron neutron capture agent comprising a ¹⁰ B containing moiety, wherein the ¹⁰ B containing moiety contains more than one ¹⁰ B atom, conjugated to a ligand having binding specificity for at least one intracellular receptor of the cells, and then irradiating the cells with neutrons.

20. A method of killing intracellular receptor containing cells comprising contacting the cells with a boron neutron capture agent comprising a ¹⁰ B containing moiety, wherein the ¹⁰ B containing moiety comprises at least one carborane residue, conjugated to a ligand having binding specificity for at least one intracellular receptor of the cells, and irradiating the cells with neutrons.

21. The method of claim 20 wherein the ¹⁰ B containing moiety is selected from the class of compounds having the following formula:

##STR12## wherein M⁺ is selected from the group consisting of an alkali metal cations and tetra alkyl ammonium;

R₁ is a group selected from the categories of compounds suitable for linking to a ligand and targeting moieties;

R₂ is a group selected from the categories of compounds suitable for linking to a ligand and targeting moieties;

m is an integer from 1 to 10; and

n is an integer from 2 to 150.

22. A method of killing intracellular receptor containing cells comprising contacting the cells with a boron neutron capture agent comprising a ¹⁰ B containing moiety, wherein the ¹⁰ B containing moiety comprises at least one carboranyl residue, conjugated to a ligand having binding specificity for at least one intracellular receptor of the cells, and irradiating the cells with neutrons.

23. The method of claim 22 wherein the ¹⁰ B containing moiety is selected from the class of compounds having the following formula:

##STR13## wherein M⁺ is selected from the group consisting of an alkali metal cations and tetra alkyl ammonium;

R₁ is a group selected from the categories of compounds suitable for linking to a ligand and targeting moieties;

R₂ is a group selected from the categories of compounds suitable for linking to a ligand and targeting moieties;

m is an integer from 1 to 10; and

n is an integer from 2 to 150.