Mutant-type bioluminescent protein, and process for producing the mutant-type bioluminescent protein

• US 6,074,859 A
• Filed: 07/08/1998
• Issued: 06/13/2000
• Est. Priority Date: 07/08/1997
• Status: Expired due to Term

• Current Assignee: Kikkoman Corporation
• Original Assignee: Kikkoman Corporation
• Inventors: Kajiyama, Naoki, Hirokawa, Kozo, Murakami, Seiji
• Primary Examiner(s): Prouty, Rebecca E.
• Assistant Examiner(s): HUTSON, RICHARD G
• Application Number:
  US09/111,752
• Time in Patent Office: 706 Days
• Field of Search: 435/8, 435/69.1, 435/71.1, 435/71.2, 435/440, 435/441, 435/189, 530/858
• US Class Current: 435/189
• CPC Class Codes: C12N 9/0069 acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
• CPC Class Codes: Y10S 530/858 insects; venom

Abstract

According to the present invention, there can be provided a bioluminescent protein, luciferase excellent in thermostability etc. and with high catalytic efficiency.

Claims

1. A bioluminescent protein having firefly luciferase activity and having a mutation in an amino acid residue corresponding to the 219-position of the Luciola cruciata luciferase.

2. The bioluminescent protein of claim 1, wherein the amino C acid residue corresponding to the 219-position of the Luciola cruciata luciferase is an isoleucine residue.

5. A bioluminescent protein having firefly luciferase activity, wherein the bioluminescent protein of claim 1 is fused to at least one other bioluminescent protein having firefly luciferase activity.

6. The bioluminescent protein of claim 5, wherein said other bioluminescent protein having firefly luciferase activity is a luciferase from Heike firefly (Luciola lateralis), American firefly (Photinus pyralis) or Genji firefly (Luciola cruciata).

7. A bioluminescent protein having firefly luciferase activity, wherein the bioluminescent protein of claim 2 is fused to at least one other bioluminescent protein having firefly luciferase activity.

8. The bioluminescent protein of claim 7, wherein said other bioluminescent protein having firefly luciferase activity is a luciferase from Heike firefly (Luciola lateralis), American firefly (Photinus pyralis) or Genji firefly (Luciola cruciata).

3. A bioluminescent protein having firefly luciferase activity and having a mutation in an amino acid residue corresponding to the 290-position of the Luciola cruciata luciferase.

4. The bioluminescent protein of claim 3, wherein the amino C acid residue corresponding to the 290-position of the Luciola cruciata luciferase is an isoleucine residue.

9. A bioluminescent protein having firefly luciferase activity, wherein the bioluminescent protein of claim 3 is fused to at least one other bioluminescent protein having firefly luciferase activity.

10. The bioluminescent protein of claim 9, wherein said other bioluminescent protein having firefly luciferase activity is a luciferase from Heike firefly (Luciola lateralis), American firefly (Photinus pyralis) or Genji firefly (Luciola cruciata).

11. A bioluminescent protein having firefly luciferase activity, wherein the bioluminescent protein of claim 4 fused to at least one other bioluminescent protein having firefly luciferase activity.

12. The bioluminescent protein of claim 11, wherein said other bioluminescent protein having firefly luciferase activity is a luciferase from Heike firefly (Luciola lateralis), American firefly (Photinus pyralis) or Genji firefly (Luciola cruciata).