Biosensor and method of manufacturing the same
First Claim
1. A biosensor comprising an electrically insulating base plate, an electrode system having a working electrode and a counter electrode formed over said base plate, a water soluble reaction layer containing at least an enzyme and an electron acceptor disposed on said electrode system, and an anionic filter formed over said reaction layer for inhibiting permeation of solid components, wherein said anionic filter is composed of a porous film made of a film-forming polymer or a fiber sheet, and an anionic polymer for inhibiting by electrostatic repulsion dispersion of said electron acceptor beyond said anionic filter, wherein said anionic polymer is supported on said porous film or fiber sheet.
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Accused Products
Abstract
Disclosed is a biosensor facilitating high accuracy quantification of a specific component in a sample solution with no adverse influence of solid substances. The biosensor comprises an electrically insulating base plate, an electrode system comprising a working electrode and a counter electrode formed on the base plate, a reaction layer containing at least one enzyme disposed on the electrode system, and an anionic filter formed on the reaction layer for inhibiting permeation of solid components.
167 Citations
11 Claims
- 1. A biosensor comprising an electrically insulating base plate, an electrode system having a working electrode and a counter electrode formed over said base plate, a water soluble reaction layer containing at least an enzyme and an electron acceptor disposed on said electrode system, and an anionic filter formed over said reaction layer for inhibiting permeation of solid components, wherein said anionic filter is composed of a porous film made of a film-forming polymer or a fiber sheet, and an anionic polymer for inhibiting by electrostatic repulsion dispersion of said electron acceptor beyond said anionic filter, wherein said anionic polymer is supported on said porous film or fiber sheet.
- 3. A biosensor comprising an electrically insulating base plate, an electrode system having a working electrode and a counter electrode formed over said base plate, a water soluble reaction layer containing at least an enzyme and an electron acceptor disposed on said electrode system, and an anionic filter formed over said reaction layer for inhibiting permeation of solid components, wherein said anionic filter is a porous film made of a mixture of a film-forming polymer and an anionic polymer for inhibiting by electrostatic repulsion dispersion of said electron acceptor beyond said anionic filter.
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5. A method of manufacturing a biosensor comprising the steps of:
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dropping a solution containing a hydrophilic polymer on an electrode system disposed on an electrically insulating base plate and drying said solution to form a hydrophilic polymer layer on said electrode system, dropping a solution containing at least one enzyme and an electron acceptor on said hydrophilic polymer layer and drying the solution to form a water soluble reaction layer on said hydrophilic polymer layer, and forming an anionic filter for covering said reaction layer said anionic filter inhibiting by electrostatic repulsion dispersion of said electron acceptor beyond said anionic filter. - View Dependent Claims (6, 7, 8, 9, 10)
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11. A method of manufacturing a biosensor comprising the steps of:
- forming a water soluble reaction layer including an enzyme, an electron acceptor and a hydrophilic polymer on an electrode system having a working electrode and a counter electrode disposed on an electrically insulating base plate, and
disposing a medium, having a film-forming polymer and an anionic polymer dissolved or dispersed therein, on said reaction layer and drying the medium to form an anionic filter wherein said anionic polymer inhibits by electrostatic repulsion dispersion of said electron acceptor beyond said anionic filter.
- forming a water soluble reaction layer including an enzyme, an electron acceptor and a hydrophilic polymer on an electrode system having a working electrode and a counter electrode disposed on an electrically insulating base plate, and
Specification