Biological seed treatment to improve emergence, vigor, uniformity and yield of sweet corn

• US 6,077,505 A
• Filed: 06/11/1997
• Issued: 06/20/2000
• Est. Priority Date: 06/11/1997
• Status: Expired due to Fees

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Regner, Kurt M., Clark, Amy D., Parke, Jennifer L.
• Primary Examiner(s): Witz, Jean C.
• Application Number:
  US08/873,167
• Time in Patent Office: 1,105 Days
• Field of Search: 424/93.4, 424/93.47, 435/252.1, 435/253.3, 435/874
• US Class Current: 424/93.4
• CPC Class Codes: A01N 63/20 Bacteria; Substances produced thereby or obtained therefrom
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12R 2001/01 Bacteria or Actinomycetales ; using bacteria or Actinomycetales
• CPC Class Codes: C12R 2001/38 Pseudomonas
• CPC Class Codes: Y10S 435/874 Pseudomonas

Abstract

A bacterial inoculant for controlling root rot and damping off in corn, and for fostering the growth of corn, is disclosed. Also disclosed are two mutant bacterial strains, Burkholderia cepacia 2J6 and Burkholderia cepacia 2J6, which foster the growth of corn and protect against fungal disease.

Claims

1. A bacterial strain of Burkholderia cepacia which is derived from B. cepacia strain AMMD and which has the corn growth fostering characteristics of mutant strains 2J6 or 2358.

2. A bacterial strain of Burkholderia cepacia which has been derived from B. cepacia strain AMMD by a screening process in which there was a selection for mutants that did not produce a yellow-green pigment, and which strain has the ability to protect corn plants against a disease caused by a fungal species without causing chlorosis in corn plants.

3. The bacterial strain of claim 2, wherein the fungal species is a member of the genus Pythium.

6. A biological inoculant for plants comprising an essentially biologically pure culture of the bacterial strain of claim 2 and a carrier.

4. A biologically pure culture of Burkholderia cepacia 2J6 (ATCC Accession No. 55982).

5. A biologically pure culture of Burkholderia cepacia AMMD 2358 (ATCC Accession No. 55983).

7. A biological inoculant for plants comprising an essentially biologically pure culture of the bacterial strain selected from the group consisting of Burkholderia cepacia 2J6 (ATCC Accession No. 55982) and Burkholderia cepacia 2358 (ATCC Accession No. 55983).

8. An agriculturally useful composition comprising a corn seed inoculated with an inoculant of an essentially biologically pure culture of a bacterial strain selected from the group consisting of Burkholderia cepacia 2J6 (ATCC Accession No. 55982) and Burkholderia cepacia 2358 (ATCC Accession No. 55983).

9. A method for controlling a fungal pathogen in a corn plant comprising inoculating the plant with an effective amount of an essentially biologically pure culture of a bacterial strain having the characteristics of bacterial strains Burkholderia cepacia 2J6 (ATCC Accession No. 55982) and Burkholderia cepacia 2358 (ATCC Accession No. 55983), wherein said characteristics include the ability to protect plants against disease caused by a fungal species without causing chlorosis in plants.

10. The method of claim 9, wherein the inoculating step comprises applying the inoculant to a seed of the plant.

11. The method of claim 9, wherein the fungal species is a member of the genus Pythium.

12. The method of claim 9, wherein the bacterial strain is selected from the group consisting of Burkholderia cepacia 2J6 (ATCC Accession No. 55982) and Burkholderia cepacia 2358 (ATCC Accession No. 55983).

13. A method for fostering the growth of a corn plant comprising inoculating the plant with an effective amount of an essentially biologically pure culture of a bacterial strain having the corn growth fostering characteristics of Burkholderia cepacia strains 2J6 or 2358.