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Kit and method for fluorescence based detection assay

  • US 6,077,669 A
  • Filed: 11/04/1997
  • Issued: 06/20/2000
  • Est. Priority Date: 11/04/1997
  • Status: Expired due to Term
First Claim
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1. A method for conducting a fluorescence detection assay to determine the presence, absence, or amount of a target analyte in a sample, said method comprising:

  • (a) producing a mixture by exposing the sample to a first reagent formulation comprising a dry, hydratable binding partner of said target analyte, said binding partner (i) being capable of producing a detectable change of fluorescence signal as the amount of target analyte changes, and (ii) being hydrated by exposure to said sample; and

    (b) exposing said mixture to a second reagent formulation or a condition, which, if said target analyte is present in the sample, then the amount of said target analyte will change.

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