Composite optical blood culture sensor

• US 6,080,574 A
• Filed: 03/04/1999
• Issued: 06/27/2000
• Est. Priority Date: 05/10/1994
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Berndt, Klaus W.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/262,520
• Time in Patent Office: 481 Days
• Field of Search: 435/288.7, 422/82.08, 422/82.09, 436/172
• US Class Current: 435/288.7
• CPC Class Codes: C12M 41/34 of gas
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404

Abstract

A culture medium and blood specimen are introduced into a sealable glass vial having a headspace gas mixture such that a change in the gas mixture composition can be monitored by a chemically sensitive material in the vial comprising a mixture of a fluorophore and a chromophore. The fluorophore exhibits a long fluorescence decay time and a fluorescence intensity that depend on a first chemical parameter, such as oxygen concentration. The chromophore exhibits an optical transmission that depends on a second chemical parameter, such as carbon dioxide concentration, the optical transmission of the chromophore changing with the second chemical parameter either within the excitation or within the emission wavelength range of the fluorophore.

Claims

1. An apparatus for detecting microorganism growth comprising:

a container comprising a culture medium, a blood specimen, and a headspace having a concentration of a gas;

a chemically sensitive material in said container for detecting microorganism growth within said container when illuminated with an intensity-modulated light, said chemically sensitive material being comprised of a first material and a second material;

said first material exhibiting a fluorescence decay time and fluorescence intensity that depend on a first chemical parameter of said gas; and

said second material exhibiting an optical transmission that depends on a second chemical parameter of said gas.

2. An apparatus according to claim 1, wherein said first chemical parameter of said gas is oxygen concentration, such that said first material exhibits a change in fluorescence decay time and fluorescence intensity in response to the change in oxygen concentration of said gas.

3. An apparatus according to claim 2, wherein said first material is a fluorophore.

4. An apparatus according to claim 3, wherein said fluorophore is tris-4,7-diphenyl-1,10-phenanthroline ruthenium (II) chloride.

5. An apparatus according to claim 1, wherein said second chemical parameter of said gas is carbon dioxide concentration, such that said second material exhibits a change in optical transmission in response to the change in carbon dioxide concentration of said gas.

6. An apparatus according to claim 5, wherein said second material is a chromophore.

7. An apparatus according to claim 6, wherein said chromophore is selected from the group consisting of bromocresol purple, m-cresol purple, nitrazine yellow, chlorophenol red or bromothymol blue.

8. An apparatus according to claim 5, wherein said second material exhibits a change in optical transmission within the excitation wavelength range or within the emission wavelength range of said first material, in response to the change in carbon dioxide concentration of said gas.

9. An apparatus according to claim 1, wherein:

said first material is a fluorophore that exhibits a change in fluorescence decay time and fluorescence intensity in response to a change in oxygen concentration of said gas; and

said second material is a chromophore that exhibits a change in optical transmission in response to a change in carbon dioxide concentration of said gas, whereby the change in fluorescence intensity emitted by said fluorophore is amplified by the optical transmission change of said chromophore.

10. An apparatus according to claim 1, further comprising:

a light source for illuminating said chemically sensitive material in said container with excitation light that is intensity-modulated at a predetermined circular modulation frequency with a predetermined modulation degree;

a photodetector for detecting fluorescence light reemerging from said chemically sensitive material;

means for measuring an AC component and a DC component of the fluorescence photocurrent from said photodetector; and

means for generating a sensor output signal based on the AC component and the DC component representing information as to whether or not microorganism growth is present within said container.

11. An apparatus according to claim 1, further comprising:

means for compiling a first set of signals corresponding to said fluorescence decay time and fluorescence intensity depending on said first chemical parameter of said gas;

means for compiling a second set of signals corresponding to said optical transmission depending on said second chemical parameter of said gas; and

means for comparing the first and second sets of signals with a corresponding predetermined data base of first and second sets of signals for known microorganisms to perform microorganism identification on a detected microorganism.

12. An apparatus for detecting microorganism growth comprising:

a container comprising a culture medium, a blood specimen, and a headspace having a concentration of a gas;

a chemically sensitive material in said container for detecting microorganism growth within said container when illuminated with an intensity-modulated light, said chemically sensitive material being comprised of a first material and a second material;

said first material is a fluorophore that exhibits a change in fluorescence decay time and fluorescence intensity in response to a change in oxygen concentration of said gas;

said second material is a chromophore that exhibits a change in optical transmission in response to a change in carbon dioxide concentration of said gas, whereby the change in fluorescence intensity emitted by said fluorophore is amplified by the optical transmission change of said chromophore;

a light source for illuminating said chemically sensitive material in said container with the intensity-modulated light at a predetermined circular modulation frequency and a predetermined modulation degree;

a photodetector for detecting fluorescence light reemerging from said chemically sensitive material;

means for measuring an AC component and a DC component of the fluorescence photocurrent from said photodetector; and

means for generating a sensor output signal based on the AC component and the DC component representing information as to whether or not microorganism growth is present within said container.