Optical detection apparatus for chemical analyses of small volumes of samples

• US 6,084,683 A
• Filed: 11/25/1998
• Issued: 07/04/2000
• Est. Priority Date: 05/28/1996
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Zeptosens AG (Bayer AG)
• Inventors: Ehrat, Markus, Herzig, Hans Peter, Volkel, Reinhard, Barnard, Steven Mark, Bruno, Alfredo Emilio, Nussbaum, Philippe
• Primary Examiner(s): Rosenberger, Richard A.
• Application Number:
  US09/194,523
• Time in Patent Office: 587 Days
• Field of Search: 356/446, 356/244, 356/246, 356/440, 435/808, 422/82.05, 422/82.08, 422/82.09
• US Class Current: 356/446
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/03 Cuvette constructions

Abstract

Optical detection apparatus for chemical analyses of small volumes of samples has light sources (21) to emit induction light, a measuring cell (7) for the sample, photoelectric sensing elements (22) to receive light coming form the sample in the measuring cell (7), as well as beam-conducting means for the induction light or the light from the sample. In the apparatus, several essentially planar, laminar components (2-7) are arranged in a sandwich structure, whereby the components (2-7) contain the light sources (21), the measuring cell (7), the photoelectric sensing elements (22), and also the beam conducting means.

Claims

1. Optical detection apparatus for chemical analyses of small volumes of samples, comprising:

a measuring cell for receiving the sample;

light sources for emitting induction light to the measuring cell;

photoelectric sensing elements for receiving light from the sample in said measuring cell; and

light conducting channels for conducting the induction light to the sample in said measuring cell and for conducting the light from the sample in said measuring cell to said photoelectric sensing elements;

wherein a plurality of essentially planar, laminar components are arranged in a sandwich structure, said components containing said measuring cell, said light sources, said photoelectric sensing elements and said light conducting channels.

2. The apparatus of claim 1, wherein said components comprise respective connections connecting said components to adjacent said components in said sandwich structure.

3. The apparatus of claim 1, wherein one of said components comprises a plurality of light conducting optical elements arranged on one plane.

4. The apparatus of claim 3, wherein one of said components comprises said light sources arranged on one plane.

5. The apparatus of claim 4, wherein one of said components comprises said photoelectric sensing elements arranged on one plane.

6. The apparatus of claim 1, wherein said light conducting channels comprise ducts.

7. The apparatus of claim 1, wherein said measuring cell is provided in one of said components that is disposed between two other of said components provided with said light sources and said photoelectric sensing elements.

8. The apparatus of claim 1, wherein:

said measuring cell, said light sources, said photoelectric sensing elements, said light conducting channels and optical elements are arranged relative to each other so as to form a plurality of separate transmitted light measuring units;

said light conducting channels comprise light inlet channels and light outlet channels;

each of said units comprises one of said light sources, one of said optical elements, one of said light conducting channels for conducting induction light to measuring cell, one of said photoelectric sensing elements and one of said light outlet channels for conducting light from the sample in said measuring cell to the one of said photoelectric sensing elements.

9. The apparatus of claim 8, wherein each of said units further comprises an additional one of said optical elements arranged between said measuring cell and said photoelectric sensing element.

10. The apparatus of claim 8, wherein each of said light inlet channels is arranged so that induction light that is emitted therefrom contacts said measuring cell essentially perpendicularly thereto.

11. The apparatus of claim 8, wherein said units are arranged so that induction light from respective said units contacts said measuring cell at different spatially divided areas of said measuring cell.

12. The apparatus of claim 1, wherein one of said components contains both said light sources and said photoelectric sensing elements arranged on one plane.

13. The apparatus of claim 12, wherein said components comprise a component which contains optical filters arranged on one plane.

14. The apparatus of claim 13, wherein said optical filters comprise micro-optical filters.

15. The apparatus of claim 13, wherein:

said measuring cell, said light sources, said photoelectric sensing elements, said light conducting channels and said optical elements are arranged relative to each other so as to form a plurality of separate transmitted light measuring units;

said light conducting channels comprise light inlet channels and light outlet channels; and

each of said units comprises one of said light sources, one of said optical elements, one of said light conducting channels for conducting induction light to measuring cell, one of said photoelectric sensing elements and one of said light outlet channels for conducting light from the sample in said measuring cell to the one of said photoelectric sensing elements.

16. The apparatus of claim 15, wherein in each of said units, said light inlet channel and said light outlet channel are inclined with respect to each other and intersect so as to have a common end that lying in an area of said measuring cell that forms both a light outlet area for induction light and a light inlet area for light from the sample.

17. The apparatus of claim 16, wherein each of said units further comprises a further photoelectric sensing element optically linked by a respective further light outlet channel to said measuring cell, said further light outlet channel being inclined with respect to said light inlet channel and intersecting with light inlet channel.

18. The apparatus of claim 16, wherein each of said units further comprises a further light source optically linked by a respective further light inlet channel to said measuring cell, said further light inlet channel being inclined with respect to said light outlet channel and intersects said light outlet channel.

19. The apparatus of claim 15, wherein in each of said units, one of said optical elements is provided between each of said light sources and said measuring cell, and a further one of said optical elements is provided between each of said photoelectric sensors and said measuring cell, said one and said further one of said optical elements being arranged on one plane.

20. The apparatus of claim 15, wherein in each of said units, one optical filter is provided between each of said light sources and said measuring cell, and a further optical filter is provided between each of said photoelectric sensors and said measuring cell, said one and said further said optical filters being arranged on one plane.

21. The apparatus of claim 15, wherein said units are arranged so that induction light from respective said units contacts said measuring cell at different spatially divided areas of said measuring cell.

22. The apparatus of claim 21, wherein said units are arranged so that said areas of said measuring cell are arranged two-dimensionally.

23. The apparatus of claim 22, wherein said measuring cell comprises a sensor layer that can be contacted by the sample and by induction light.

24. The apparatus of claim 23, wherein said sensor layer comprises different zones which are sensitive to respective different analytes.

25. The apparatus of claim 24, wherein said optical elements comprise micro-optical elements.

26. The apparatus of claim 25, wherein said micro-optical elements are refractive or diffractive or hybrid micro-optical elements.

27. The apparatus of claim 1, wherein said measuring cell comprises a plurality of sample chambers.

28. The apparatus of claim 1, where+in said light sources comprise light-emitting diodes.

29. The apparatus of claim 1, wherein one of said components comprises control and evaluation equipment.