Methods of ultrasound imaging using echogenically persistent contrast agents

• US 6,086,540 A
• Filed: 10/07/1997
• Issued: 07/11/2000
• Est. Priority Date: 10/07/1997
• Status: Expired due to Fees

• Current Assignee: Molecular Biosystems, Inc (Alliance Pharmaceutical Corporation)
• Original Assignee: Molecular Biosystems, Inc (Alliance Pharmaceutical Corporation)
• Inventors: Miller, Jeffrey John, Kuvelas, Mary Therese, Bonneville, Daniel Aron, Killam, Anne Louise, Greener, Yigal
• Primary Examiner(s): Jaworski, Francis J.
• Assistant Examiner(s): Imam, Ali M.
• Application Number:
  US08/946,342
• Time in Patent Office: 1,008 Days
• Field of Search: 600/458, 600/454, 600/437, 600/441, 600/443, 600/447
• US Class Current: 600/458
• CPC Class Codes: A61K 49/223 Microbubbles, hollow microspheres, free gas bubbles, gas microspheres

Abstract

The present invention relates to a method of imaging using multiple doses of an echogenically persistent contrast agent. In particular, it relates to the use of ultrasound energy to eliminate residual echogenicity attributed to the first dose of contrast agent prior to administering a second dose of contrast agent for the purpose of acquiring more images.

Claims

1. A method of performing a multiple dose ultrasound imaging study of a site within a mammalian subject comprising the steps of:

a) generating an ultrasound image of the site after administering a first dose of a microbubble ultrasound contrast agent by applying ultrasound at a first energy level;

b) eliminating echogenicity from the first dose of the microbubble ultrasound contrast agent by applying ultrasound at a second energy level, wherein the second energy level is greater than the first energy level; and

c) generating an ultrasound image of the site after administration of a second dose of a microbubble ultrasound contrast agent.

2. The method of claim 1, wherein the microbubble contrast agent comprises protein shelled microspheres.

3. The method of claim 2, wherein the microsphere contains air.

4. The method of claim 2, wherein the microsphere contains air and perfluoropentane.

5. The method of claim 2, wherein the microsphere contains air and perfluorohexane.

6. The method of claim 1, wherein the microbubble contrast agent comprises an emulsion.

7. The method of claim 6, wherein the emulsion comprises perfluorocarbon.

8. The method of claim 1, wherein the site of imaging includes a microcapillary bed.

9. The method of claim 1, wherein the ultrasound energy applied in step a) is intermittent, and the ultrasound energy applied in step b) is continuous.

10. The method of claim 1, wherein the ultrasound energy applied in step a) is at a first power level, and the ultrasound energy applied in step b) is at a second power level, wherein the second power level is greater than the first power level.

11. The method of claim 1, wherein the ultrasound energy applied in step a) is in B-mode, and the ultrasound energy applied in step b) is in color doppler mode.

12. The method of claim 1, wherein the ultrasound energy applied in step a) is at a first frequency, and the ultrasound energy applied in step b) is at a second frequency, wherein the second frequency is less than the first frequency.