Nucleic acid mediated electron transfer
First Claim
1. A single-stranded nucleic acid containing at least one electron donor moiety and at least one electron acceptor moiety, said electron donor moiety and said electron acceptor moiety being covalently attached to said nucleic acid.
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Abstract
The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
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Citations
16 Claims
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1. A single-stranded nucleic acid containing at least one electron donor moiety and at least one electron acceptor moiety, said electron donor moiety and said electron acceptor moiety being covalently attached to said nucleic acid.
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2. A composition comprising a first single stranded nucleic acid comprising an electrode and a second single stranded nucleic acid comprising a covalently attached electron transfer moiety.
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3. A method of detecting a target sequence in a nucleic acid sample comprising:
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a) applying a first input signal to a hybridization complex comprising said target sequence, which if present, is hybridized to at least a first nucleic acid, wherein said hybridization complex has a covalently attached electron donor moiety and a covalently attached electron acceptor moiety; and b) detecting electron transfer between said electron donor moiety and said electron acceptor moiety as an indication of the presence or absence of said target sequence. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification