Monoclonal antibodies for human mesenchymal stem cells

• US 6,087,113 A
• Filed: 05/19/1998
• Issued: 07/11/2000
• Est. Priority Date: 06/18/1991
• Status: Expired due to Term

• Current Assignee: Mesoblast International Srl
• Original Assignee: Case Western Reserve University
• Inventors: Caplan, Arnold I., Haynesworth, Stephen E.
• Primary Examiner(s): Johnson, Nancy A.
• Application Number:
  US09/081,913
• Time in Patent Office: 784 Days
• Field of Search: 435/7.1, 435/7.2, 435/7.21, 530/388.2, 530/388.7
• US Class Current: 435/7.1
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61F 2/30756 Cartilage endoprostheses A61F2/3603 takes precedence
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61L 2430/10 for reconstruction of tendons or ligaments
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3843 Connective tissue
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61L 31/005 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: C12N 2501/23 Interleukins [IL]
• CPC Class Codes: C12N 2501/2301 Interleukin-1 (IL-1)
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2533/18 Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0663 Bone marrow mesenchymal stem cells (BM-MSC)

Abstract

The invention relates to isolated human mesenchymal stem cells, a method for isolating, purifying, and culturally expanding human mesenchymal stem cells (i e. mesenchymal stem cells or "MSCs"), and characterization of (including by cytokine expression profiling) and uses, particularly research reagent, diagnostic and therapeutic uses for such cells. The stem cells can be culture expanded without differentiating. Further disclosed are monoclonal antibodies specific for human mesenchymal stem cells and the monoclonal hybridoma cell lines (ATCC nos. 10743-10745) that synthesize and secrete these monospecific antibodies, and uses of the monoclonal antibodies for diagnostic and/or therapeutic purposes.

Claims

1. A process for recovering human mesenchymal stem cells from a cell mixture derived from human bone marrow comprising contacting the cell mixture with a monoclonal antibody which selectively binds to an antigen on human mesenchymal stem cells and recovering antibody bound cells from said cell mixture.

3. The process of claim 1 wherein said human mesenchymal stem cells are isolated, purified, culture-expanded human mesenchymal stem cells.

2. A process for separating a cell population containing human mesenchymal stem cells from a cell mixture derived from human bone marrow comprising contacting the cell mixture with a monoclonal antibody which selectively binds to an antigen on human mesenchymal stem cells and separating antibody bound cells from said cell mixture.

4. The process of claim 2 wherein said human mesenchymal stem cells are isolated, purified, culture-expanded human mesenchymal stem cells.

5. A method for determining the presence of human mesenchymal stem cells in a cell mixture derived from human bone marrow, comprising:

contacting the cell mixture with a monoclonal antibody that selectively binds human mesenchymal stem cells; and

detecting the presence of said antibody.

6. The method of claim 5 wherein said human mesenchymal stem cells are culture-expanded.