Methods for the modulation of lignification levels

• US 6,087,557 A
• Filed: 10/06/1992
• Issued: 07/11/2000
• Est. Priority Date: 10/06/1992
• Status: Expired due to Fees

• Current Assignee: Salk Institute for Biological Studies, University Of Oldenberg
• Original Assignee: Salk Institute for Biological Studies
• Inventors: Clausen, Monika, Megnet, Roland, Elkind, Yonatan, Lamb, Christopher J., Doerner, Peter
• Primary Examiner(s): McElwain, Elizabeth F.
• Application Number:
  US07/957,941
• Time in Patent Office: 2,835 Days
• Field of Search: 435/172.3, 435/69.1, 435/71.1, 435/91.1, 435/240.4, 435/257.3, 435/419, 435/468, 800/205, 800/298, 800/295, 800/278
• US Class Current: 800/278
• CPC Class Codes: C12N 15/8255 involving lignin biosynthesis
• CPC Class Codes: C12N 9/88 Lyases (4.)

Abstract

In accordance with the present invention, there are provided methods to modulate plant lignification. For example, the level of the enzyme phenylalanine ammonia-lyase (PAL), a key enzyme in the production of lignin precursors, can be increased or decreased, as desired, to increase or decrease lignification levels. Alternatively, the level of lignin precursors in the plant can be modulated either by enhancing the plant'"'"'s ability to produce such precursors, or by causing the compounds which are required for the production of lignin precursors to undergo alternative chemical conversions (which prevent the incorporation of such compounds into lignin by diverting the lignin precursors to other chemical pathways). In addition, there is provided a method for producing plants having modified lignin composition and content, by converting a portion of the cinnamic acid in said plant to styrene. In another embodiment, there is provided a method for producing styrene and/or styrene derivatives, by contacting cinnamic acid or a cinnamic acid derivative with PAD under decarboxylation conditions.

Claims

1. A method for producing PAD in a plant comprising:

transforming said plant with a PAD gene, and

expressing said PAD gene in said plant.

2. A method according to claim 1, wherein said plant is transformed with a construct comprising said PAD gene under the control of a suitable promoter.