DNA typing by mass spectrometry with polymorphic DNA repeat markers
First Claim
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1. A method for multiplexing the identification of more than one DNA tandem nucleotide repeat region from more than one DNA tandem nucleotide repeat loci, comprising:
- obtaining more than one nucleic acid extension product by extending one or more primers complementary to sequences flanking the DNA tandem nucleotide repeat region, and;
determining the masses of more than one nucleic acid extension product simultaneously by mass spectrometry,wherein the nucleic acid extension products have overlapping allelic mass ranges.
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Abstract
The present invention is related to the fields of genetic mapping and genetic identity detection, including forensic identification and paternity testing. This invention is more specifically directed to the use of mass spectrometry to detect length variation in DNA nucleotide sequence repeats (including variants of common alleles), such as microsatellites and short tandem repeats, and to DNA sequences provided as primers for the analysis of DNA tandem nucleotide repeat polymorphisms at specific loci on specific chromosomes.
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30 Claims
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1. A method for multiplexing the identification of more than one DNA tandem nucleotide repeat region from more than one DNA tandem nucleotide repeat loci, comprising:
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obtaining more than one nucleic acid extension product by extending one or more primers complementary to sequences flanking the DNA tandem nucleotide repeat region, and; determining the masses of more than one nucleic acid extension product simultaneously by mass spectrometry, wherein the nucleic acid extension products have overlapping allelic mass ranges. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method for multiplexing the identification of more than one DNA tandem nucleotide repeat region from more than one DNA tandem nucleotide repeat loci, comprising:
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obtaining more than one nucleic acid amplification product by amplifying two or more primers complementary to sequences flanking the DNA tandem nucleotide repeat region; and determining the masses of more than one nucleic acid amplification product simultaneously by mass spectrometry, wherein the nucleic acid extension products have overlapping allelic mass ranges. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification