Cleavage agents
First Claim
Patent Images
1. A thermostable structure-specific nuclease having an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 102, 107, 130 and 132.
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Abstract
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to improved cleavage means for the detection and characterization of nucleic acid sequences. Structure-specific nucleases derived from a variety of thermostabe organisms are provided. These structure-specific nucleases are used to cleave target-dependent cleavage structures, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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Citations
24 Claims
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1. A thermostable structure-specific nuclease having an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 102, 107, 130 and 132.
- View Dependent Claims (2)
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3. A recombinant DNA vector comprising DNA having a nucleotide sequence encoding a structure-specific nuclease, said nucleotide sequence selected from the group consisting of SEQ ID NO:
- 101, 106, 129 and 131.
- View Dependent Claims (4, 5)
- 6. A purified Pyrococcus woesii FEN-1 endonuclease, wherein said Pyrococcus woesii FEN-1 endonuclease is encoded by the plasmid pTrc99-PWFEN-1 (ACTT 207094).
- 8. An isolated oligonucleotide comprising at least a portion of the plasmid pTrc99-PWFEN-1 (ACTT 207094), said portion encoding Pyrococcus woesii FEN-1 endonuclease.
- 12. A recombinant DNA vector comprising an isolated oligonucleotide, said oligonucleotide comprising at least a portion of the plasmid pTrc99-PWFEN-1 (ACTT 207094), said portion encoding Pyrococcus woesii FEN-1 endonuclease.
- 15. An isolated oligonucleotide comprising a gene encoding a Pyrococcus woesii FEN-1 endonuclease having a molecular weight of about 38.7 kilodaltons, wherein said Pyrococcus woesii FEN-1 endonuclease comprises the plasmid pTrc-99FWFEN-1 (ACTT 207094).
- 19. A recombinant DNA vector comprising DNA having a nucleotide sequence encoding a Pyrococcus woesii FEN-1 endonuclease having a molecular weight of about 38.7 kilodaltons, and wherein said Pyrococcus woesii FEN-1 endonuclease comprises the plasmid pTrc-99FWFEN-1 (ACTT 207094).
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22. An isolated oligonucleotide encoding Pyrococcus woesii FEN-1 endonuclease, said oligonucleotide comprising a sequence fully complementary to at least 15 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NOS:
- 116-119.
- View Dependent Claims (23, 24)
Specification