Device and method for performing spectral measurements in flow cells with spatial resolution

• US 6,091,502 A
• Filed: 12/23/1998
• Issued: 07/18/2000
• Est. Priority Date: 12/23/1998
• Status: Expired due to Term

• Current Assignee: Perkinelmer Health Sciences Incorporated (Perkinelmer Incorporated)
• Original Assignee: Micronics Inc. (Sony Group Corp.)
• Inventors: Altendorf, Eric, Weigl, Bernhard H.
• Primary Examiner(s): Font, Frank G.
• Assistant Examiner(s): LAUCHMAN, LAYLA G
• Application Number:
  US09/219,677
• Time in Patent Office: 573 Days
• Field of Search: 356/416, 356/418, 356/419, 356/326, 356/244, 349/62
• US Class Current: 356/416
• CPC Class Codes: G01N 15/14 Optical investigation techniques, e.g. flow cytometry
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: G01N 30/0005 Field flow fractionation

Abstract

A device and method for performing spectral measurements in flow cells with spatial resolution using a variable transmission optical filter having at least two areas with different optical properties. Light from a light source passes through the variable transmission filter to a flow cell containing a sample to be analyzed. The resultant light pattern is sensed by a detecting means, which analyzes the spectral properties of the sample within the flow cell.

Claims

1. A device for analyzing the spectral properties of a sample, comprising:

a light source;

a microfluidic channel for holding a flowing sample to be analyzed;

a variable transmission optical filter having at least two areas with different optical properties;

and detecting means capable of measuring light intensity with at least one dimensional resolution;

wherein when light from said light source is directed at said flowing sample within said channel and through said optical filter and is received by said detecting means, said detecting means analyzes the spectral properties of the flowing sample.

2. The device of claim 1, wherein said optical filter is positioned between said light source and said channel.

3. The device of claim 1, wherein said optical filter is positioned between said channel and said detecting means.

4. The device of claim 1, wherein said optical filter comprises a linear variable filter.

5. The device of claim 1, wherein said optical filter comprises a filter array.

6. The device of claim 1, wherein said optical filter is comprised of a plurality of bandpass filters.

7. The device of claim 1, wherein said detecting means includes a CCD camera.

8. The device of claim 1, further comprising a second variable transmission optical filter having at least two areas with different optical properties, with said sample containing channel being positioned between said optical filters.

9. The device of claim 1, wherein said optical filter is constructed from a combination of filters selected from a group comprised of bandpass filters, longpass filters, polarizing filters and neutral density filters.

10. The device of claim 1, wherein said optical filter comprises a programmable LCD screen having variable optical transmission.

11. The device of claim 1, wherein said optical filter comprises a programmable LCD screen having variable optical reflection.

12. The device of claim 1, wherein said optical filter comprises a printed filter array.

13. The device of claim 1, wherein said sample holding channel comprises a flow cell.

14. The device of claim 13, wherein said sample holding chamber comprises a plurality of flow cells.

15. The device of claim 1, wherein said optical filter is comprised of at least two areas having different optical properties which are printed onto a substrate by a computer addressable ink jet type printer.

21. The device of claim 1 wherein said microfluidic channel is contained within a T-Sensor.

16. A method of analyzing the optical properties of a sample, comprising the steps of:

locating a flowing sample to be analyzed within a microfluidic measurement cell;

directing a light source at said measurement cell;

filtering the light from said light source using a variable transmission optical filter having at least two areas with different optical properties;

receiving the light from said light source which has passed through said optical filter and said flowing sample within said measurement cell at a detection means and analyzing the spectral properties of said sample.

17. The method of claim 16, wherein said optical filter is positioned between said light source and said measurement cell.

18. The method of claim 16, wherein said optical filter is positioned between said measurement cell and said detection means.

19. The method of claim 16, wherein said detection means comprises a CCD camera.

22. The method of claim 16, wherein said optical filter comprises a linear variable filter.

23. The method of claim 22, wherein said microfluidic measurement cell is positioned within a T-Sensor.

20. A method of making an optical filter, comprising the steps of:

selecting an optically transparent substrate;

and attaching to said substrate at least one optically active substance having at least two areas of different optical properties;

wherein said method of attaching comprises printing said areas of different optical properties with an ink jet printer.