Method and device for moving molecules by the application of a plurality of electrical fields

• US 6,093,296 A
• Filed: 11/19/1997
• Issued: 07/25/2000
• Est. Priority Date: 02/28/1990
• Status: Expired due to Term

• Current Assignee: Monogram Biosciences, Inc. (Laboratory Corporation Of America Holdings)
• Original Assignee: Aclara Biosciences, Inc (Laboratory Corporation Of America Holdings)
• Inventors: Soane, David S., Soane, Zoya M.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): STARSIAK, JOHN S
• Application Number:
  US08/974,372
• Time in Patent Office: 979 Days
• Field of Search: 204/600, 204/601, 204/602, 204/603, 204/604, 204/605, 204/450, 204/451, 204/452, 204/453, 204/454, 204/455, 435/287.2, 435/288.5, 435/288.6, 435/288.3, 435/288.4, 435/288.7, 422/68.1, 422/81, 422/82
• US Class Current: 204/451
• CPC Class Codes: B01D 57/02 by electrophoresis treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01F 33/3031 using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00853 Employing electrode arrangements
• CPC Class Codes: B01J 2219/00912 by electrophoresis
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: C07K 1/26 Electrophoresis
• CPC Class Codes: G01N 27/44752 Controlling the zeta potential, e.g. by wall coatings
• CPC Class Codes: G01N 27/44773 Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
• CPC Class Codes: G03F 7/0002 Lithographic processes using patterning methods other than those involving the exposure to radiation, e.g. by stamping

Abstract

Devices and methods are disclosed for moving charged molecules through a medium by the application of a plurality of electrical fields of sufficient strength and applied for sufficient amounts of time so as to move the charged molecules through the medium. The devices although preferably small in size, preferably generate large numbers (100 or more) of electrical fields to a movement area which preferably contains a liquid buffered or gel medium. Mixtures of charged molecules are pulled through the gel by the force of the electrical fields. The fields are preferably activated simultaneously or sequentially one after another at various speeds to create complex force field distributions or moving field waves along the separation medium. Charged molecules capable of moving quickly through the gel will be moved along by the faster moving field waves and be separated from slower moving molecules. The fields can be activated by computer software and can be used to move molecules away from and toward each other to obtain rapid and complex chemical synthesis, sequencing or reaction protocols.

Claims

1. A method for moving charged molecules under electrophoretic conditions through a medium in a movement area by the application of a plurality of electric fields employing a device comprised of:

a device for moving charged particles through a medium employing an electric field, said device comprising;

an electrically non-conductive solid support polymeric card having an upper surface;

a main trench in said solid support having capillary dimensions and extending downward from said upper surface;

a plurality of branch trenches having capillary dimensions on said main trench;

a medium in said trenches; and

a plurality of electrodes positioned to be in contact with said medium and to control electrophoretic movement in said branch trenches in accordance with the size, shape and charge of said charged particles;

said method comprising;

moving electrophoretically in at least one of said branch trenches or said main trench by means of said electric fields in said medium first charged molecules into contact with second charged molecules;

whereby said charged molecules are brought into contact and react.

2. The method of claim 1, wherein said first and second charged molecules react in a synthesis or sequencing reaction.

3. The method of claim 2, wherein said reaction is the sequencing of DNA.

4. The method of claim 2, wherein said reaction is the synthesis of DNA.

5. The method of claim 2, wherein said reaction is the sequencing of a protein.

6. The method of claim 2, wherein said reaction is the synthesizing of a protein.