Miniaturized planar columns in novel support media for liquid phase analysis

• US 6,093,362 A
• Filed: 02/11/1999
• Issued: 07/25/2000
• Est. Priority Date: 10/19/1994
• Status: Expired due to Term

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Mittelstadt, Laurie S., Bek, Fritz, Swedberg, Sally A., Kaltenbach, Patrick, Witt, Klaus E.
• Primary Examiner(s): Silbaugh, Jan H.
• Assistant Examiner(s): McDowell, Suzanne E.
• Application Number:
  US09/249,531
• Time in Patent Office: 530 Days
• Field of Search: 264/400, 264/156, 264/219, 264/220, 264/238, 264/328.1, 264/328.5, 156/272.8, 156/217, 156/292, 156/290, 156/273.3, 156/272.2, 156/379.6, 156/252, 156/257, 156/258, 73/61.52, 210/198.2, 210/656, 422/70, 95/82, 95/85, 96/101, 96/105, 96/107, 427/133
• US Class Current: 264/400
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/5055 Hinged, e.g. opposable surfaces
• CPC Class Codes: B01L 7/54 using spatial temperature gradients
• CPC Class Codes: B29C 65/02 by heating, with or without pressure
• CPC Class Codes: B29C 66/54 Joining several hollow-preforms, e.g. half-shells, to form hollow articles, e.g. for making balls, containers; Joining several hollow-preforms, e.g. half-cylinders, to form tubular articles
• CPC Class Codes: B29C 66/549 said hollow-preforms being interconnected during their moulding process, e.g. by a hinge
• CPC Class Codes: B29L 2031/756 Microarticles, nanoarticles
• CPC Class Codes: G01N 2030/0035 electrical field
• CPC Class Codes: G01N 2030/285 electrically driven carrier
• CPC Class Codes: G01N 2030/3007 : same temperature for whole column
• CPC Class Codes: G01N 2030/3015 : temperature gradients along column
• CPC Class Codes: G01N 2030/303 : using peltier elements
• CPC Class Codes: G01N 27/44704 : Details; Accessories
• CPC Class Codes: G01N 27/44708 : Cooling
• CPC Class Codes: G01N 27/44721 : by optical means
• CPC Class Codes: G01N 27/44773 : Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
• CPC Class Codes: G01N 27/44791 : Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: G01N 30/02 : Column chromatography
• CPC Class Codes: G01N 30/60 : Construction of the column
• CPC Class Codes: G01N 30/6047 : with supporting means; Holders
• CPC Class Codes: G01N 30/6052 : body
• CPC Class Codes: G01N 30/606 : with fluid access or exit ports
• CPC Class Codes: G01N 30/6095 : Micromachined or nanomachined, e.g. micro- or nanosize
• CPC Class Codes: Y10T 156/1064 : Partial cutting [e.g., grooving or incising]

Abstract

Miniaturized planar column devices are described for use in liquid phase analysis, the devices comprising microstructures fabricated by laser ablation in a variety of novel support substrates. Devices formed according to the invention include associated laser-ablated features required for function, such as analyte detection means and fluid communication means. Miniaturized columns constructed under the invention find use in any analysis system performed on either small and/or macromolecular solutes in the liquid phase and may employ chromatographic, electrophoretic, electrochromatographic separation means, or any combination thereof.

Claims

1. A method of forming a plurality of identical miniaturized column devices, comprising the steps of:

(a) providing a master support body having a substantially planar interior surface, wherein the master support body is comprised of a material other than silicon and silicon dioxide;

(b) forming a master copy of a miniaturized column device by;

forming a microchannel directly in the interior surface of the master support body;

(c) forming a mold insert by;

(i) coating the interior surface of the master copy with a layer of a metal;

(ii) depositing a metal thereon to fill the microchannel formed in the interior surface; and

(iii) separating the mold insert from the master support body, whereby a mold insert is provided; and

(d) preparing additional miniaturized column devices identical to the master copy by filling the mold insert with a suitable polymeric or ceramic material, whereby a miniaturized column device thus formed can be covered with a plate over the microchannel of the additional miniaturized column device to form a device having a microchannel for a fluid to pass therethrough.

2. A method of forming a plurality of identical miniaturized channel devices, comprising the steps of:

(a) providing a master support body having a substantially planar surface, wherein the master support body is comprised of a material other than silicon and silicon dioxide;

(b) forming a master copy of a miniaturized channel device by forming a microchannel directly in the surface of the master support body;

(c) forming a mold insert by filling the microchannel formed in the surface of the master support body with metal and separating the mold insert from the master support body, the mold insert having a first substantially planar surface corresponding to the substantially planar surface of the master support body;

(d) preparing additional miniaturized channel devices identical to the master copy by filling the mold insert with a suitable polymeric or ceramic material; and

(e) removing each replica from the mold insert and arranging a plate on the first planar surface of each replica, to form each miniaturized channel device;

wherein each miniaturized channel device comprises a substrate comprising a replica and having first and second substantially planar surfaces, said substrate having a microchannel in the first planar surface and a plate arranged over the first planar surface, said plate in combination with the microchannel defining an elongate compartment for fluid to pass therethrough.

3. The method of claim 2 further comprising molding replicas of a plate having a microchannel thereon and arranging a replica of said plate on the first planar surface of each replica formed using the mold insert.

4. The method of claim 2 further comprising forming a microchannel on another planar surface on the opposite side of the substantially planar surface of the master support body, forming a mold insert using the master support body;

preparing replicas of the master support body using the mold insert; and

arranging a plate on each side of each replica of the master support body to form each miniaturized channel device.

5. The method of claim 2 further comprising forming a coat of metal on the substantially planar surface of the master copy with a metal and depositing metal on said coat of metal to fill the microchannel to form the mold insert.

6. The method of claim 2 further comprising coating the substantially planar surface of the master copy with a layer of a first metal and depositing a second metal thereon to fill the microchannel.

7. The method of claim 2 further comprising forming a plurality of apertures in the master support body, said apertures being open for fluid communication with the microchannel.

8. The method of claim 2 further comprising forming the microchannel by laser ablation.

9. A method of forming a plurality of identical miniaturized channel devices, comprising the steps of:

(a) providing a master support body having first and second halves, said halves comprising substantially planar surfaces for forming microchannels thereon, said planar surfaces comprising interior surfaces, wherein the master support body is comprised of a material other than silicon and silicon dioxide;

(b) forming a master copy of a miniaturized channel device by;

(i) forming a first microchannel directly in the interior surface of the first half of the master support body; and

(ii) forming a second microchannel directly in the interior surface of the second half of the master support body, wherein the second microchannel is arranged to provide the mirror image of the first microchannel;

(c) forming a mold insert from each half of the master support body by filling the microchannel formed in the interior surface with metal and separating the mold insert from each half of the master support body, whereby a mold insert is provided for each half;

(d) preparing additional miniaturized channel devices identical to the master copy by filling the mold inserts with a suitable polymeric or ceramic material; and

(e) removing each replica from the mold insert and arranging the first half on the second half of each replica, to form each miniaturized channel device;

wherein each miniaturized channel device comprises a first half and a second half each having a microchannel facing the other, said microchannel in the first half in combination with the microchannel in the second half defining an elongate compartment for the passage of a fluid therethrough.