Substrate for detecting UL9 helicase activity
First Claim
1. A UL9 substrate comprising:
- a first strand including a UL9 herpes replication origin sequence and a first single stranded tail 3'"'"' relative to the herpes replication origin sequence; and
a second strand including a sequence complementary to the UL9 herpes replication origin sequence.
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Abstract
A UL9 substrate for detecting helicase activity in a UL9 protein is provided. In one embodiment, the substrate includes a first strand including a herpes replication origin sequence and a first single stranded tail; and a second strand including a sequence complementary to the herpes replication origin sequence. In one variation, the first single stranded tail is 3'"'"' relative to the herpes replication origin sequence. The second strand may optionally further include a single stranded tail. The single stranded tail on the second strand may be 3'"'"' or 5'"'"' relative to the sequence complementary to the herpes replication origin sequence. In another variation, the first single stranded tail is 5'"'"' relative to the herpes replication origin sequence. The second strand may optionally further include a single stranded tail. The single stranded tail on the second strand may be 3'"'"' or 5'"'"' relative to the sequence complementary to the herpes replication origin sequence. The UL9 substrate may optionally further include at least one detectable marker attached to at least one of the first and second strands. The UL9 substrate may also optionally be attached to a solid support.
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Citations
45 Claims
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1. A UL9 substrate comprising:
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a first strand including a UL9 herpes replication origin sequence and a first single stranded tail 3'"'"' relative to the herpes replication origin sequence; and a second strand including a sequence complementary to the UL9 herpes replication origin sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A UL9 substrate comprising:
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a first strand including a UL9 herpes replication origin sequence and a first single stranded tail 3'"'"' relative to the herpes replication origin sequence; a second strand including a sequence complementary to the UL9 herpes replication origin sequence; and at least one detectable marker attached to at least one of the first and second strands. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. An immobilized UL9 substrate comprising:
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a first strand including a UL9 herpes replication origin sequence and a first single stranded tail 3'"'"' relative to the herpes replication origin sequence; a second strand including a sequence complementary to the UL9 herpes replication origin sequence; and a solid support to which one of the first and second sequences is attached. - View Dependent Claims (23, 24, 25, 26, 27)
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28. A UL9 substrate comprising:
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a first strand including a UL9 herpes replication origin sequence and a first single stranded tail 5'"'"' relative to the herpes replication origin sequence; and a second strand including a sequence complementary to the UL9 herpes replication origin sequence and a single stranded tail 3'"'"' relative to the sequence complementary to the herpes replication origin sequence. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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39. An immobilized UL9 substrate comprising:
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a first strand including a UL9 herpes replication origin sequence and a first single stranded tail 5'"'"' relative to the herpes replication origin sequence; and a second strand including a sequence complementary to the UL9 herpes replication origin sequence and a single stranded tail 3'"'"' relative to the sequence complementary to the herpes replication origin sequence; and a solid support to which one of the first and second sequences is attached. - View Dependent Claims (40, 41, 42, 43, 44, 45)
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Specification