Multi-slide assembly including slide, frame and strip cap, and methods thereof

• US 6,096,562 A
• Filed: 10/27/1997
• Issued: 08/01/2000
• Est. Priority Date: 10/27/1997
• Status: Expired due to Term

• Current Assignee: Nalge NUNC International Corporation (Perspecta, Inc.)
• Original Assignee: Nalge NUNC International Corporation (Perspecta, Inc.)
• Inventors: Khan, Abdul Wahid, Nanda, Susan K. W., Williamson, Kathleen R., Matthai, Hoyt E., Bunn, Kenneth E., Whittlinger, Keith O., Sullivan, Barbara Myszkiewicz
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US08/958,521
• Time in Patent Office: 1,009 Days
• Field of Search: 422/58, 422/99, 422/102, 435/283.1, 435/287.1, 435/287.2, 435/288.3, 435/288.4, 436/518, 436/527, 436/531, 436/809
• US Class Current: 436/518
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: B01L 3/50855 using modular assemblies of strips or of individual wells
• CPC Class Codes: G01N 2001/2846 Cytocentrifuge method
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

A multi-slide assembly includes various components that alone or in combination facilitate testing of test samples with minimal manipulation of assay components. Moreover, the various device components permit centrifugation, culturing and analysis of each test sample to be performed in the same assembly. A frame retains a plurality of reaction vessel assemblies between a pair of opposing channels that are configured to slidably receive the opposing ends of each reaction vessel assembly. A strip cap seals the openings in each reaction vessel using cap members having sealing rings circumscribing the external walls thereof to form compression seals with internal walls of the reaction vessels. Furthermore, in a method of making a multi-well slide assembly, an ultrasonic welding process removably bonds a plurality of wells to a slide plate to provide an adequate seal therebetween during centrifugation, yet still enable separation thereof by an operator.

Claims

1. A strip cap for use in sealing an opening in at least one reaction vessel having an internal wall defining the opening, the strip cap including:

(a) a cap member having an external wall, a mating portion of which is configured to abut the internal wall of the reaction vessel around a perimeter of the opening; and

(b) a sealing ring circumscribing the external wall of the cap member, the sealing ring configured to form a compression seal with the internal wall of the reaction vessel.

2. The strip cap of claim 1, further comprising a second sealing ring circumscribing the external wall of the cap member in spaced apart relationship with the first sealing ring, the first and second sealing rings defining a vent channel therebetween, the vent channel placing the reaction vessel in fluid communication with the atmosphere when the strip cap is partially tilted from the reaction vessel.

3. The strip cap of claim 2, further comprising a reinforcement ring circumscribing an internal wall of the cap member intermediate the first and second sealing rings.

4. The strip cap of claim 1, further comprising a plurality of ribs, each extending between bottom and side portions of the internal wall of the cap member.

5. The strip cap of claim 1, wherein the mating portion of the external wall is cylindrical.

6. The strip cap of claim 5, wherein the mating portion of the external wall is tapered.

7. The strip cap of claim 6, wherein the external wall further comprises a bottom portion extending generally perpendicular to the mating portion thereof, and an annular bevel joining the mating and bottom portions of the external wall.

8. The strip cap of claim 1, further comprising a plurality of cap members disposed in a linear arrangement and joined by a strip member, and each including a sealing ring configured to mate with one of a linear arrangement of reaction vessels.

9. The strip cap of claim 8, wherein the strip member includes a flange portion circumscribing each cap member and configured to overlap an end portion of the internal wall of each cell culture member proximate the opening thereof.

10. The strip cap of claim 8, wherein the plurality of reaction vessels comprise a multi-well slide including a plurality of wells coupled to a slide plate, wherein each reaction vessel includes one of the plurality of wells.

11. The strip cap of claim 8, wherein the plurality of cap members, the plurality of sealing rings, and the strip member are molded as an integral unit.

12. The strip cap of claim 11, wherein the plurality of cap members, the plurality of sealing rings, and the strip member are formed from a flexible material.

13. The strip cap of claim 12, wherein the plurality of cap members, the plurality of sealing rings, and the strip member are formed of a material selected from the group consisting of low density polyethylene, high density polyethylene, polypropylene, and a thermoplastic elastomer.

14. A multi-well slide assembly, comprising:

(a) a slide plate;

(b) a plurality of wells coupled to the slide plate, each well including an internal wall defining an opening to the well; and

(c) a strip cap, including;

(1) a strip member;

(2) a plurality of cap members coupled to the strip member, each cap member having an external wall, a mating portion of which is configured to abut the internal wall of one of the plurality of wells around a perimeter of the opening thereto; and

(3) a plurality of sealing rings, each sealing ring circumscribing the external wall of one of the plurality of cap members, and each sealing ring configured to form a compression seal with the internal wall of one of the plurality of wells.

15. The multi-well slide assembly of claim 14, further comprising a second plurality of sealing rings, each circumscribing the external wall of one of the plurality of cap members in spaced apart relationship with the first sealing ring thereon, wherein the first and second sealing rings on each cap member define a vent channel therebetween, the vent channel placing the well in which the cap member is disposed in fluid communication with the atmosphere when the cap member is partially tilted from the well.

16. The multi-well slide assembly of claim 14, further comprising a biological or chemical substance housed within each of the plurality of wells, the biological or chemical substance selected from the group consisting of a cell culture, a biochemical reagent, a biological substance, and combinations thereof.

17. The multi-well slide assembly of claim 16, further comprising a transport medium housed within each of the plurality of wells.

18. A frame for use in retaining a plurality of reaction vessels, each reaction vessel of the type including opposing ends, the frame comprising;

(a) a pair of opposing channels facing in different directions from one another, the pair of opposing channels including first and second ends and configured to slidably receive the opposing ends of each reaction vessel; and

(b) first and second retaining mechanisms respectively disposed proximate the first and second ends of the pair of opposing channels and configured to retain each reaction vessel within the pair of opposing channels.

19. The frame of claim 18, wherein each reaction vessel includes opposing engagement members defining the opposing ends such that the pair of opposing channels engage the engagement members of each reaction vessel.

20. The frame of claim 18, wherein each reaction vessel includes a plate on which is defined the opposing ends such that the pair of opposing channels engage the plate of each reaction vessel.

21. The frame of claim 20, wherein the first retaining mechanism includes a first stop member restricting movement of the reaction vessels beyond the first end of the pair of opposing channels.

22. The frame of claim 21, further comprising an access opening defined proximate the first end of the pair of opposing channels.

23. The frame of claim 22, wherein each channel has a longitudinal axis and includes opposing upper and lower walls respectively configured to restrict movement of the plate of each reaction vessel in a direction perpendicular to a surface of the walls, the upper wall of each channel including a retaining portion and an access portion, the access portion at least partially defining the access opening, and the access portion of the upper wall of each channel extending toward the first end of the channel from a point intermediate the first and second ends of the channel, and extending away from the lower wall of the channel at an acute angle relative to the longitudinal axis of the channel.

24. The frame of claim 22, wherein the first retaining mechanism further includes a detent extending from the first stop member toward the second end of each channel.

25. The frame of claim 24, wherein the detent is spaced from the lower wall of each channel at substantially the same distance as the retaining portion of the upper wall of the channel.

26. The frame of claim 25, wherein the plate of each reaction vessel has a thickness, and wherein the detent and the retaining portion of the upper wall of each channel are spaced from the lower wall at distance substantially equal to the thickness of the plate of each reaction vessel.

27. The frame of claim 25, wherein each reaction vessel has a width defined along the longitudinal axis of each channel when the reaction vessel is slidably received between the pair of opposing channels, and wherein the detent includes an end spaced from the second end of the channel at a distance that is substantially the same as a sum of the widths of the plurality of reaction vessels.

28. The frame of claim 20, wherein the frame includes a lower support member coupled to and extending between the pair of opposing channels, the lower support member configured to support each reaction vessel in a direction generally perpendicular to the plate thereof.

29. The frame of claim 28, wherein the frame defines an upper access aperture extending between the pair of opposing channels and between the first and second retaining mechanisms.

30. The frame of claim 28, wherein the lower support member defines at least one access aperture through which is visible at least one of the plurality of reaction vessels.

31. The frame of claim 30, wherein the access aperture is defined by a void in the lower support member.

32. The frame of claim 30, wherein the lower support member includes a transparent material forming a visual access aperture.

33. The frame of claim 18, wherein the second retaining mechanism includes a stop member restricting movement of the reaction vessels beyond the second end of the pair of opposing channels.

34. The frame of claim 18, wherein each reaction vessel includes a multi-well slide including a plurality of wells.

35. The frame of claim 34, wherein the frame is configured to retain four multi-well slides, each including four wells on each slide.

36. The frame of claim 18, wherein the frame has a footprint that is substantially similar to that of a microwell plate, and has a height that is no greater than that of a microwell plate.

37. The frame of claim 18, wherein each reaction vessel is selected from the group consisting of a cell culture vessel, a multi-well slide, a flask, a dish, a culture tube, a wafer, a slide, a plate, a disc and a microarrayed surface.

38. The frame of claim 18, wherein the pair of opposing channels face one another.

39. An assembly, comprising:

(a) a plurality of reaction vessels, each including opposing ends; and

(b) a frame retaining the plurality of reaction vessels, the frame including;

(1) a pair of opposing channels including first and second ends and configured to slidably receive the opposing ends of the plurality of reaction vessels; and

(2) first and second retaining mechanisms respectively disposed proximate the first and second ends of the pair of opposing channels and configured to retain each reaction vessel within the pair of opposing channels.

40. The assembly of claim 39, wherein each reaction vessel includes a multi-well slide including a plurality of wells defined on a slide plate, and wherein the opposing ends of each multi-well slide are defined on the slide plate such that the pair of opposing channels engage the slide plate of each multi-well slide.

41. The assembly of claim 40, wherein:

(a) the first retaining mechanism includes a first stop member restricting movement of the multi-well slides beyond the first end of the pair of opposing channels;

(b) the second retaining mechanism includes a stop member restricting movement of the multi-well slides beyond the second end of the pair of opposing channels;

(c) each channel has a longitudinal axis and includes opposing upper and lower walls respectively configured to restrict movement of the slide plate of each multi-well slide in a direction perpendicular to a surface of the walls, the upper wall of each channel including a retaining portion and an access portion, the access portion at least partially defining an access opening for the frame, and the access portion of the upper wall of each channel extending toward the first end of the channel from a point intermediate the first and second ends of the channel, and extending away from the lower wall of the channel at an acute angle relative to the longitudinal axis of the channel; and

(d) the first retaining mechanism further includes a detent extending from the first stop member toward the second end of each channel, the detent spaced from the lower wall of each channel at substantially the same distance as the retaining portion of the upper wall of the channel.

42. The assembly of claim 41, wherein:

(a) the slide plate of each multi-well slide has a width and a thickness;

(b) the detent and the retaining portion of the upper wall of each channel are spaced from the lower wall at distance substantially equal to the thickness of the slide plate of each multi-well slide; and

(c) the detent includes an end spaced from the second end of the channel at a distance that is substantially the same as a sum of the widths of the slide plates of the plurality of multi-well slides.

43. The assembly of claim 40, wherein the frame includes a lower support member coupled to and extending between the pair of opposing channels, the lower support member configured to support each multi-well slide in a direction generally perpendicular to the slide plate thereof, the lower support member defining at least one access aperture through which is visible a test sample disposed in one of the plurality of wells on one of the plurality of multi-well slides.

44. The assembly of claim 40, wherein the plurality of wells on each multi-well slide are removably coupled to the respective slide plates via ultrasonic welds.

45. The assembly of claim 40, wherein each well on the plurality of multi-well slides includes an internal wall defining an opening to the well, the assembly further comprising a strip cap coupled to at least one of the multi-well slides, the strip cap including:

(a) a strip member;

(b) a plurality of cap members coupled to the strip member, each cap member having an external wall, a mating portion of which is configured to abut the internal wall of one of the plurality of wells around a perimeter of the opening thereto; and

(c) a plurality of sealing rings, each sealing ring circumscribing the external wall of one of the plurality of cap members, and each sealing ring configured to form a compression seal with the internal wall of one of the plurality of wells.

46. The assembly of claim 39, wherein the pair of opposing channels face one another.

47. A reaction vessel, comprising:

(a) a slide plate;

(b) a sidewall member including a sidewall including an end and having a thickness, and an energy director including a base coupled to an end of the sidewall and having a width; and

(c) an ultrasonically-welded and controllably-weakened bond removably securing the sidewall member to the slide plate, the bond comprising a fused junction between the slide plate and the sidewall member and formed of substantially the entire energy director, wherein the slide plate and sidewall member form a mating surface having a width between the width of the energy director and the thickness of the sidewall.

48. The reaction vessel of claim 47, wherein the sidewall member comprises an upper structure defining in cooperation with the slide plate a plurality of wells.

49. The reaction vessel of claim 47, wherein the slide plate and the sidewall member are formed of chemically-resistant plastic.