Nucleotide compounds including a rigid linker
First Claim
1. A nucleoside/tide compound having the structure
wherein;
NUC is a nucleoside/tide having a nucleobase portion B;
L is a rigid linkage;
S is a spacer; and
LB/LG is a member of a linkage pair or a label;
whereinNUC is attached to L through B such that when B is a purine, L is attached to the 8-position of the purine, when B is 7-deazapurine, L is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, L is attached to the 5-position of the pyrimidine; and
L has the structure ##STR9## wherein each of n, o and p are integers ranging from 0 to 3, and the sum of n, o and p is at least 2; and
each of W, X, Y and Z is selected from the group consisting of --CH and N, including substituted forms thereof.
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Accused Products
Abstract
A nucleoside/tide compound having the structure
NUC--L--S--LB/LG
is described wherein NUC is a nucleoside/tide having a nucleobase portion B, L is a rigid linkage, S is a spacer; and LB/LG is a member of a linkage pair or a label. NUC is attached to L through B such that when B is a purine, L is attached to the 8-position of the purine, when B is 7-deazapurine, L is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, L is attached to the 5-position of the pyrimidine. In an important aspect of the invention, L has the structure ##STR1## wherein each of n, o and p are integers ranging from 0 to 3, and the sum of n, o and p is at least 2, and each of W, X, Y and Z is selected from the group consisting of carbon and nitrogen. The invention further includes polynucleotide compounds comprising the nucleoside/tide, and primer extension methods utilizing the nucleoside/tide, particularly when used in combination with certain mutant polymerase enzymes.
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Citations
22 Claims
- 1. A nucleoside/tide compound having the structure
- space="preserve" listing-type="equation">NUC--L--S--LB/LG
wherein; NUC is a nucleoside/tide having a nucleobase portion B; L is a rigid linkage; S is a spacer; and LB/LG is a member of a linkage pair or a label;
whereinNUC is attached to L through B such that when B is a purine, L is attached to the 8-position of the purine, when B is 7-deazapurine, L is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, L is attached to the 5-position of the pyrimidine; and L has the structure ##STR9## wherein each of n, o and p are integers ranging from 0 to 3, and the sum of n, o and p is at least 2; and each of W, X, Y and Z is selected from the group consisting of --CH and N, including substituted forms thereof. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
- 10. The compound of claim 9 wherein n is 1 or 2.
- 11. The compound of claim 1 wherein LB/LG is a member of a linkage pair.
- 12. The compound of claim 11 wherein the member of the linkage pair is amine.
- 13. The compound of claim 12 wherein the amine is a primary amine.
- 14. The compound of claim 1 wherein LB/LG is a label.
- 15. The compound of claim 14 wherein the label is a xanthene-type dye.
- 16. The compound of claim 15 wherein the xanthene-type dye is a rhodamine or a fluorescein dye.
- 17. The compound of claim 1 wherein --L-- has the structure ##STR10##
- 18. The compound of claim 1 wherein --L-- has the structure
- space="preserve" listing-type="equation">--C.tbd.C--C.tbd.C--
Specification