Human immunoglobulin V.sub.H gene segments and DNA fragments containing the same

• US 6,096,878 A
• Filed: 03/27/1996
• Issued: 08/01/2000
• Est. Priority Date: 05/10/1993
• Status: Expired due to Fees

• Current Assignee: Japan Tobacco Inc.
• Original Assignee: Japan Tobacco Inc.
• Inventors: Honjo, Tasuku, Matsuda, Fumihiko
• Primary Examiner(s): BURKE, JULIE ELAINE
• Application Number:
  US08/545,809
• Time in Patent Office: 1,588 Days
• Field of Search: 536/23.1, 536/23.53, 536/23.51, 435/91.1
• US Class Current: 536/23.53
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002

Abstract

Novel human immunoglobulin V_H segments and DNA fragments containing the same are disclosed. The DNA fragment according to the present invention is the fragment having a size of about 800 kbp which is shown in FIG. 1. The human immunoglobulin V_H segments according to the present invention are contained in the fragment of this DNA fragment of about 800 kbp, and there are 50 novel segments. The base sequences of these :segments are shown in the Sequence Listing. The present invention also provides DNA fragments which contain two or more of these V_H segments.

Claims

1. An isolated polynucleotide comprising the following nucleic acid sequences of (a) through (f) in 5'"'"' to 3'"'"' order:

(a) a nucleic acid sequence of a portion of a human genome isolable from;

(i) a yeast artificial chromosome clone Y6 which is isolable from a transformant identified by an international deposit number FERM BP-4271;

or

(ii) a yeast artificial chromosome clone Y24 which is isolable from a transformant identified by an international deposit number FERM BP-4274;

(b) a nucleic acid sequence of a portion of a human genome isolable from a yeast artificial chromosome clone Y21 which is isolable from a transformant identified by an international deposit number FERM BP-4273, wherein said nucleic acid sequence lacks the 5'"'"' terminal sequence of said portion of a human genome so isolable from the clone Y21 that duplicates the 3'"'"' terminal sequence of the nucleic acid sequence of (a);

(c) a nucleic acid sequence of a portion of a human genome isolable from a cosmid vector clone M118 which is isolable from a transformant identified by an international deposit number FERM BP-4278, wherein said nucleic acid sequence lacks the 5'"'"' terminal sequence of said portion of a human genome so isolable from the clone M118 that duplicates the 3'"'"' terminal sequence of the nucleic acid sequence of (b);

(d) a nucleic acid sequence of a portion of a human genome isolable from a cosmid vector clone M84 which is isolable from a transformant identified by an international deposit number FERM BP-4277, wherein said nucleic acid sequence lacks the 5'"'"' terminal sequence of said portion of a human genome so isolable from the clone M84 that duplicates the 3'"'"' terminal sequence of the nucleic acid sequence of (c);

(e) a nucleic acid sequence of a portion of a human genome isolable from a cosmid vector clone M131 which is isolable from a transformant identified by an international deposit number FERM BP-4279, wherein said nucleic acid sequence lacks the 5'"'"' terminal sequence of said portion of a human genome so isolable from the clone M131 that duplicates the 3'"'"' terminal sequence of the nucleic acid sequence of (d); and

(f) a nucleic acid sequence of a portion of a human genome isolable from a cosmid vector clone 3-31 which is isolable from a transformant identified by an international deposit number FERM BP-4276, wherein said nucleic acid sequence lacks the 5'"'"' terminal sequence of said portion of a human genome so isolable from the clone 3-31 that duplicates the 3'"'"' terminal sequence of the nucleic acid sequence of (e),

wherein each of said portions of a human genome which is so isolable from the clone Y6, Y24, Y21, M118, M84, M131 and 3-31 respectively is in a relative position in a human genome as shown in FIG. 1.

2. The polynucleotide of claim 1 wherein the polynucleotide has the restriction pattern and organization shown in FIG. 1.

3. The polynucleotide of claim 1 wherein the nucleic acid sequence of a portion of a human genome isolable from the clone Y6 comprises the nucleic acid sequences of SEQ ID NOS:

32 through 64;

the nucleic acid sequence of a portion of a human genome isolable from the clone Y24 comprises the nucleic acid sequences of SEQ ID NOS;

32 through 64;

the nucleic acid sequence of a portion of a human genome isolable from the clone Y21 comprises the nucleic acid sequences of SEQ ID NOS;

15 through 34;

the nucleic acid sequence of a portion of a human genome isolable from the clone M118 comprises the nucleic acid sequences of SEQ ID NOS;

14 and 15;

the nucleic acid sequence of a portion of a human genome isolable from the clone M84 comprises the nucleic acid sequences of SEQ ID NOS;

9 through 13;

the nucleic acid sequence of a portion of a human genome isolable from the clone M131 comprises the nucleic acid sequences of SEQ ID NOS;

8 and 9; and

the nucleic acid sequence of a portion of a human genome isolable from the clone 3-31 comprises the nucleic acid sequences of SEQ ID NOS;

6 through 8.

4. An isolated polynucleotide comprising the following nucleic acid sequences of (a) and (b) in 5'"'"' to 3'"'"' order:

(a) a nucleic acid sequence of a portion of a human genome isolable from a yeast artificial chromosome clone Y103 which is isolable from a transformant identified by an international deposit number FERM BP-4275; and

(b) a nucleic acid sequence of a portion of a human genome isolable from a yeast artificial chromosome clone Y20 which is isolable from a transformant identified by an international deposit number FERMBP-4272, wherein said nucleic acid sequence lacks the 5'"'"' terminal sequence of said portion of a human genome so isolable from the clone Y20 that duplicates the 3'"'"' terminal sequence of the nucleic acid sequence of (a),

wherein each of said portions of a human genome which is so isolable from the clone Y103 and Y20 respectively is in a relative position in a human genome as shown in FIG. 1.

5. The polynucleotide of claim 4 wherein the polynucleotide has the restriction pattern and organization shown in FIG. 1.

6. The polynucleotide of claim 4 wherein the nucleic acid sequence of a portion of a human genome isolable from the clone Y103 comprises the nucleic acid sequences of SEQ ID NOS:

1 through 5, and the nucleic acid sequence of a portion of a human genome isolable from the clone Y20 comprises the nucleic acid sequences of SEQ ID NOS;

1 through 4.

7. An isolated polynucleotide of a portion of a human genome comprising the following nucleic acid sequences of (1) through (28) in 5'"'"' to 3'"'"' order:

(1) SEQ ID NO;

64;

SEQ ID NO;

61;

(3) SEQ ID NO;

59;

(4) SEQ ID NO;

53;

(5) SEQ ID NO;

51;

(6) SEQ ID NO;

49;

(7) SEQ ID NO;

48;

(8) SEQ ID NO;

46;

(9) SEQ ID NO;

45;

(10) SEQ ID NO;

43;

(11) SEQ ID NO;

39;

(12) SEQ ID NO;

35;

(13) SEQ ID NO;

34;

(14) SEQ ID NO;

33;

(15) SEQ ID NO;

31;

(16) SEQ ID NO;

30;

(17) SEQ ID NO;

28;

(18) SEQ ID NO;

26;

(19) SEQ ID NO;

23;

(20) SEQ ID NO;

21;

(21) SEQ ID NO;

20;

(22) SEQ ID NO;

18;

(23) SEQ ID NO;

15;

(24) SEQ ID NO;

13;

(25) SEQ ID NO;

11;

(26) SEQ ID NO;

9; and

(27) SEQ ID NO;

8; and

(28) SEQ ID NO;

7;

wherein an intervening nucleic acid sequence appears between each of said adjacent nucleic acid sequences of (1) through (28), said intervening nucleic acid sequence being that found in;

a) a yeast artificial chromosome clone which is isolable from a transformant identified by an international deposit number selected from the group consisting of FERM BP-4271, FERM BP-4273, and FERM BP-4274;

or

b) a cosmid vector clone which is isolable from a transformant identified by an international deposit number selected from the group consisting of FERM BP-4276, FERM BP-4277, FERM BP-4278, and FERM BP-4279.

8. An isolated polynucleotide of a portion of a human genome comprising the following nucleic acid sequences of (1) through to (5) in 5'"'"' to 3'"'"' order:

(1) SEQ ID NO;

5 (2) SEQ ID NO;

4;

(3) SEQ ID NO;

3;

(4) SEQ ID NO;

2;

(5) SEQ ID NO;

1;

wherein an intervening nucleic acid sequence appears between each of said adjacent nucleic acid sequences of (1) through (5), said intervening nucleic acid sequence being that found in a yeast artificial chromosome clone which is isolable from a transformant identified by an international deposit number selected from the group consisting of FERM BP-4272 and FERM BP-4275.