Lepidopteran insect resistant transgenic potato plants

• US 6,100,456 A
• Filed: 03/16/1992
• Issued: 08/08/2000
• Est. Priority Date: 03/16/1992
• Status: Expired due to Fees

• Current Assignee: Board of Trustee Operating Michigan State University
• Original Assignee: The Board of Trustees of Michigan State University (Michigan State University)
• Inventors: Cheng, Jianping, Sticklen, Masomeh B.
• Primary Examiner(s): Smith, Lynette R. F.
• Assistant Examiner(s): Nelson, Amy J.
• Application Number:
  US07/851,509
• Time in Patent Office: 3,067 Days
• Field of Search: 800/205, 800/DIG. 42, 800/279, 800/302, 800/317.2, 536/23.71, 435/172.3, 435/240.4, 435/320.1, 435/69.1, 435/419, 435/468, 435/469, 935/67
• US Class Current: 800/317.2
• CPC Class Codes: C12N 15/8286 for insect resistance
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

Lepidopteran insect resistant transformed potato plants are described. In particular, the transformation of potato plants with five to ten copies of a translational fusion encoding the 68 kD lepidopteran-specific toxin from Bacillus thuringiensis var. kurstaki HD-73 and neomycin phosphotransferase II is described. The transgenic potato plants were resistant to the tobacco hornworm, a lepidopteran insect which is susceptible to Bacillus thuringiensis var. kurstaki toxin.

Claims

1. A transformed potato plant comprising DNA as set forth in SEQ ID NO:

1 which encodes a Bacillus thuringiensis endotoxin, wherein five to ten copies of the DNA are integrated into the genome of the plant and wherein the endotoxin is expressed at a level such that the plant is resistant to Lepidopteran insects.

2. The transformed potato plant of claim 1 wherein the potato plant prior to transformation is Solanum tuberosum FL 1607.

3. The transformed potato plant of claim 1 wherein the DNA has been transformed into the plant using Agrobacterium tumefaciens as a vector.

4. The transformed potato plant of claim 1 wherein the DNA is linked to a marker DNA which allows selection for the transformed plant.

5. The transformed potato plant of claim 4 wherein the marker DNA encodes neomycin phosphotransferase II.

6. The transformed potato plant of claim 1 wherein the potato plant prior to transformation is Solanum tuberosum FL1607, wherein the DNA has been transformed into the plant using Agrobacterium tumefaciens as a vector, and wherein the DNA is linked to a marker DNA which allows selection for the transformed plant.

7. The transformed potato plant of claim 6 wherein the marker DNA encodes neomycin phosphotransferase II.

8. The transformed potato plant of claim 1 wherein the DNA is operably linked to a polyadenylation encoding DNA region.

9. The transformed potato plant of claim 8 wherein the polyadenylation encoding DNA region comprises Tom PI 3'"'"' and NOS 3'"'"'.

10. A method for producing a transformed potato plant which is resistant to Lepidopteran insects comprising:

(a) transforming potato plants with DNA encoding a Bacillus thuringiensis endotoxin as set forth in SEQ ID NO;

1; and

(b) selecting a transformed potato plant, wherein five to ten copies of the DNA are integrated into the genome of the plant and wherein the endotoxin is expressed at a level such that the plant is resistant to Lepidopteran insects.

11. The method of claim 10 wherein the transformed potato plant prior to transformation is Solanum tuberosum FL 1607.

12. The method of claim 10 wherein the DNA is transformed into the plant using Agrobacterium tumefaciens as a vector.

13. The method of claim 10 wherein the DNA is linked to a marker DNA which allows selection for the transformed plant.

14. The method of claim 13 wherein the marker DNA encodes neomycin phosphotransferase II.

15. The method of claim 10 wherein the transformed potato plant prior to transformation is Solanum tuberosum FL1607, wherein the DNA is transformed into the plant using Agrobacterium tumefaciens as a vector, and wherein the DNA is linked to a marker DNA which allows selection for the transformed plant.

16. The method of claim 15 wherein the marker DNA encodes neomycin phosphotransferase II.

17. The method of claim 10 wherein the DNA is operably linked to a polyadenylation encoding DNA region.

18. The method of claim 17 wherein the polyadenylation encoding DNA region comprises Tom PI 3'"'"' and NOS 3'"'"'.