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Hybridization assay signal enhancement

  • US 6,103,474 A
  • Filed: 10/30/1998
  • Issued: 08/15/2000
  • Est. Priority Date: 10/21/1996
  • Status: Expired due to Fees
First Claim
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1. A method comprising steps ofadding a homopolymeric region to a target nucleic acid analyte, which target nucleic acid analyte has a target sequence;

  • contacting the analyte under hybridizing conditions with a multiplicity of reporter probes, said probes comprising a signal region and an oligonucleotide sequence, said oligonucleotide sequence being complementary to said analyte homopolymeric region, so as to form a stable hybrid of multiple reporter probes with said analyte homopolymeric region; and

    forming an analyte;

    capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions.

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