Oxidoreductase activity of manganic porphyrins

• US 6,103,714 A
• Filed: 07/24/1996
• Issued: 08/15/2000
• Est. Priority Date: 09/20/1994
• Status: Expired due to Term

• Current Assignee: Goodnow Capital, L.L.C.
• Original Assignee: Duke University
• Inventors: Liochev, Stefan I., Fridovich, Irwin
• Primary Examiner(s): MacMillan, Keith D.
• Application Number:
  US08/685,529
• Time in Patent Office: 1,483 Days
• Field of Search: 514/185
• US Class Current: 514/185
• CPC Class Codes: A61K 31/409 having four such rings, e.g. porphine derivatives, bilirubin, biliverdine hemin, hematin A61K31/555
• CPC Class Codes: A61K 31/4439 containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole nicotine A61K31/465
• CPC Class Codes: A61K 31/555 containing heavy metals, e.g. hemin, hematin, melarsoprol
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention relates, in general, to porphyrin complexes and, in particular, to methods of using porphyrin complexes to impart oxidoreductase activity.

Claims

1. A method of effecting oxidoreduction in a patient comprising administering to said patient a metallic porphyrin complex having substituents on the methine carbons of the porphyrin, in an effective, nontoxic amount, so that said complex is reduced by a reductant present in said patient and reoxidized by O₂^- present in said patient.

2. The method according to claim 1 wherein said substituents on said methine carbons are such that said metallic porphyrin complex, in vivo, has superoxide dismutase activity.

3. The method according to claim 2 wherein said substituents comprise a pyridyl or phenyl group.

4. The method according to claim 1 wherein said metal complex protects a superoxide dismutase (SOD) competent strain of E. coli against paraquat.

5. The method according to claim 1 wherein said metal complex protects a SOD-null strain of E. coli against dissolved oxygen.

6. The method of claim 1 wherein said metal or manganese, iron, copper, cobalt or nickle.

7. The method of claim 6 wherein said metal is manganese.

8. The method according to claim 1 wherein said reductant is NADH or NADPH.

9. The method according to claim 1 wherein said reductant is GSH.

10. The method according to claim 1 wherein said substitutents on said methine carbons are the same.

11. The method according to claim 10 wherein each of said methine carbons bears a substituent.

12. The method according according to claim 10 wherein said metal is manganese, iron, copper, cobalt or nickle.

13. The method according to claim 12 wherien said metal is manganese.

14. The method according to claim 10 wherein said reductant is NADH or NADPH.

15. The method according to claim 10 wherein said reductant is GSH.

16. The method according to claim 11 wherein said metal is manganese, iron, copper, cobalt or nickle.

17. The method according to claim 16 wherein said metal is manganese.

18. The method according to claim 11 wherein said reductant is NADH or NADPH.

19. The method according to claim 11 wherein said reductant is GHS.