DNA amplification and subtraction techniques

• US 6,107,023 A
• Filed: 06/17/1988
• Issued: 08/22/2000
• Est. Priority Date: 06/17/1988
• Status: Expired due to Term

• Current Assignee: Illumina Incorporated
• Original Assignee: Genelabs Technologies, Inc. (GlaxoSmithKline PLC)
• Inventors: Kim, Jungsuh, Reyes, Gregory R.
• Primary Examiner(s): Zitomer, Stephanie W.
• Application Number:
  US07/208,512
• Time in Patent Office: 4,449 Days
• Field of Search: 435/6.91, 435/199, 435/252.3, 435/91.1, 435/91.2, 436/501, 536/27, 536/28, 536/24.2, 536/24.3, 536/24.33, 536/25.4, 935/4, 935/6, 935/16, 935/19, 935/77, 935/78
• US Class Current: 435/6.16
• CPC Class Codes: C12N 15/1096 cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
• CPC Class Codes: C12Q 1/6809 Methods for determination or identification of nucleic acids involving differential detection
• CPC Class Codes: C12Q 2525/155 incorporating/generating a new priming site
• CPC Class Codes: C12Q 2539/101 Subtraction analysis
• CPC Class Codes: C12Q 2563/131 the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin

Abstract

A method of isolating genomic or RNA-derived duplex fragments which are unique to one of two fragment mixtures. The fragments in positive-source and negative-source mixtures are separately equipped with end linkers, and each mixture is amplified by successive primed-strand replications, using a single primer which is homologous to the associated linker. The second-source linker is biotinylated, and the fragments in this mixture are hybridized in molar excess with the fragments in the positive-source mixture. DNA species which are not hybridized with the biotinylated species, i.e., species that are unique to the positive-source mixture, are isolated after removal of hybridized species by affinity chromatography. Also disclosed is a method of amplifying a mixture of DNA fragments by repeated linker/primer replication.

Claims

1. A method of isolating duplex DNA fragments which are present in a mixture of different-sequence duplex DNA fragments derived from a positive source, but absent from a mixture of different-sequence duplex DNA fragments derived from a negative source, said method comprising

attaching a double-strand linker to the positive-source fragments, and separately, to the negative-source fragments, by ligating the linker to both strands of said positive-source and negative-source fragments, at both ends of said positive-source and negative-source fragments,

amplifying the number of each linker-carrying fragment in each fragment mixture by successively repeating the steps of (i) denaturing the fragments to produce single fragment strands with linker regions at each strand end, (ii) hybridizing the single strands with a single-strand primer whose sequence is complementary to the linker region at one end of each strand, to form a strand/primer complex, and (iii) converting the strand/primer complexes to double-strand fragments in the presence of polymerase and deoxynucleotides,

denaturing the amplified fragments in the two amplified fragment mixtures and hybridizing the denatured fragments in the two mixtures under conditions in which the linker regions associated with the positive-source strands do not hybridize with the linker regions associated with the negative-source strands, and

selectively isolating DNA species which are not hybridized with DNA fragment strands from the negative source.

2. The method of claim 1, wherein the fragment mixtures are derived from messenger RNA isolated from positive and negative sources, respectively, and the duplex fragments in each mixture are produced by converting the messenger RNA species in each mixture to double-strand cDNA fragments.

3. The method of claim 1, wherein the positive source is a body-fluid sample from an individual infected with a selected microorganism, and the negative-source is the same body fluid sample obtained from a noninfected individual.

4. The method of claim 1, wherein the fragments in each mixture have blunt ends, and the linkers attached to fragments have blunt ends which form, in a blunt end-to-blunt end linker dimer, a restriction site which can be cleaved with a selected restriction endonuclease.

5. The method of claim 4, wherein the linker attached to the positive-source fragments and the linker attached to the negative-sequence fragments have non-homologous sequences.

6. The method of claim 4, wherein the linker attached to the positive-source fragment has an internal restriction site at which the fragment can be cut by a selected restriction endonuclease, and the method further includes cloning isolated duplex DNA fragments, after digestion with said endonuclease, at a suitable vector cloning site.

7. The method of claim 1, wherein said hybridizing is carried out in a molar excess of the negative-source fragments.

8. The method of claim 1, wherein the negative-source fragments are biotinylated, prior to said hybridizing, and said removing includes removing biotinylated duplex fragments by affinity chromatography.

9. The method of claim 8, wherein the linkers attached to the negative-source fragments have an internal restriction site which can be cleaved with a selected restriction endonuclease to produce a sticky end, and the negative-source fragments are biotinylated by digesting the amplified fragments with the selected endonuclease, and filling in the sticky end with polymerase in the presence of biotinylated deoxynucleotides.

10. The method of claim 1, which further includes amplifying the number of each of the isolated DNA species by successively repeating the steps of (i) denaturing the fragments to produce single fragment strands, (ii) hybridizing the single strands with a single-strand primer whose sequence is complementary to the primer region at one end of each strand, to form a strand/primer complex, and (iii) converting the strand/primer complexes to double-strand fragments in the presence of polymerase and deoxynucleotides.

11. The method of claim 10, for further isolating duplex fragments which are unique to the positive-source mixture, which further includes denaturing the negative-source mixture and the amplified isolated DNA species, and hybridizing the denatured fragments in the two mixtures under conditions in which the linker regions associated with the positive-source strands do not hybridize with the linker regions associated with the negative-source strands, and

selectively isolating DNA species which are not hybridized with DNA fragment strands from the second mixture.

12. A method of amplifying a mixture of different sequence duplex DNA fragments, comprising

attaching a double-strand linker to the fragments, by ligating the linkers to both strands of the fragments, at both fragment ends,

denaturing the fragments to produce single fragment strands with linker regions at both strand ends,

hybridizing the single strands with a primer whose sequence is complementary to a linker region on each fragment strand, to form strand/primer complexes,

converting the strand/primer complexes to double-strand fragments in the presence of polymerase and deoxynucleotides, and

repeating said denaturing, hybridizing, and converting steps until a desired degree of amplification is achieved.

13. The method of claim 12, wherein the fragments are genomic fragments.

14. The method of claim 12, wherein the fragments are produced by converting messenger RNA species to double-strand cDNA fragments.