Uni-directional cell stretching device

• US 6,107,081 A
• Filed: 02/05/1999
• Issued: 08/22/2000
• Est. Priority Date: 02/05/1999
• Status: Expired due to Fees

• Current Assignee: United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration
• Original Assignee: United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration
• Inventors: Feeback, Daniel L., Clarke, Mark S. F.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/245,950
• Time in Patent Office: 564 Days
• Field of Search: 435/286.1, 435/286.2, 435/284.1, 435/289.1, 73/788, 73/807, 73/815, 73/816, 73/817, 73/826, 73/837, 73/856
• US Class Current: 435/284.1
• CPC Class Codes: A61B 5/1108 of excised organs, e.g. muscle preparations
• CPC Class Codes: A61B 5/4519 Muscles A61B5/389, A61B5/224 take precedence
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
• CPC Class Codes: G01N 2203/0089 Biorheological properties
• CPC Class Codes: G01N 3/08 by applying steady tensile or compressive forces G01N3/28 takes precedence

Abstract

The present invention relates to an apparatus and method for applying various degrees of linear, mechanical loads on mammalian tissues, and in particular, for effecting linear stretching of tissue and simulating changes in hydrostatic pressures encountered during tissue contraction in vivo. The apparatus is useful for the study of mechanical loading in human tissue, and specifically, for permitting the evaluation of the effects of mechanical loading of skeletal or cardiac tissue and of the effects of removal of mechanical loading due to inactivity or the like, and the subsequent reapplication of load to these tissues.

Claims

1. A uni-directional cell stretching device capable of mimicking linear tissue loading profiles, comprising:

a tissue culture vessel;

an actuator assembly, including a relatively fixed structure and an axially translatable ram within said vessel;

at least one elastic strip having one end portion removably connected to said ram and another, opposite end portion removably connected to said relatively fixed structure, said elastic strip being coated with an extra-cellular matrix; and

driving means for axially translating said ram relative to said relatively fixed structure, and for axially translating said one end portion of said elastic strip affixed to said ram relative to said another, opposite end portion, for longitudinally stretching said at least one elastic strip.

2. The device of claim 1, wherein said driving means comprises a pump, a sealed piston chamber receiving a proximal end portion of said ram, and means for conducting fluid under pressure from said pump to said piston chamber for driving said ram.

3. The device of claim 2, wherein said chamber is filled with a sterile fluid.

4. The device of claim 1, wherein said at least one strip is connected to said ram by removable support pins extending within a cylindrical tube support.

5. The device of claim 1, wherein said at least one strip is a silicon strip.

6. The device of claim 1, wherein said tissue culture vessel contains tissue cultured cells grown and stretched on said at least one strip.

7. The device of claim 1, wherein said tissue culture vessel contains a tissue culture medium.

8. The device of claim 1, wherein said pump is microprocessor-controlled.

9. The device of claim 7, wherein said pump controls the amount of load placed upon cells.

10. The device of claim 1, wherein said ram moves bi-directionally in a single plane in an interval or continuous mode.

11. The device of claim 2, wherein said ram is driven by injecting a sterile fluid into said chamber using said pump.

12. The device of claim 1, wherein said driving means comprises a controllable motor and gearing means drivingly connecting said motor with said ram.

13. The device of claim 12, wherein said motor comprises a linear stepper motor and wherein said gearing means comprises a screw drive assembly.

19. A method of applying linear load profiles on cells in culture, comprising the step of:

placing said cells on said strip in the uni-directional cell stretching device of claim 1.

20. The method of claim 19, wherein said cells are selected from the group consisting of skeletal muscle cells, cardiac muscle cells, endothelial cells, vascular smooth muscle cells and chondrocytes.

14. A uni-directional cell stretching device capable of mimicking linear loading profiles, comprising:

a ram having distal and proximal end portions and a piston chamber portion receiving said ram proximal end portion;

at least one elastic strip, wherein said at least one strip is connected to said distal end portion of said ram;

driving means for axially translating said ram while said strip is connected thereto, and for stretching said strip longitudinally by movement of said ram;

a tissue culture vessel containing said strip;

optical ports formed in said culture vessel; and

medium exchange/medium sampling ports communicating through said culture vessel.

15. The device of claim 14, wherein said driving means comprises a linear stepper motor and screw drive assembly.

16. The device of claim 15, wherein said stepper motor/screw drive assembly controls the amount of load placed upon cells on said strip.

17. The device of claim 14, wherein said tissue culture vessel contains a tissue culture medium, further comprising a piezoelectric-heating jacket arranged for heating said medium.

18. The device of claim 14, wherein said optical ports allow real-time viewing of cells.

21. A method of applying linear load profiles on cells in culture, comprising the step of:

placing said cells in the uni-directional cell stretching device of claim 14.

22. The method of claim 21, wherein said cells are selected from the group consisting of skeletal muscle cells, cardiac muscle cells, endothelial cells, vascular smooth muscle cells and chondrocytes.

23. The method of claim 21, comprising the steps of axially translating said ram while said strip is connected thereto for stretching said strip longitudinally by movement of said ram;

and removing said strip with said cells.

24. The method of claim 23, further comprising the step of utilizing said strip as a planar viewing surface for examination of said cells.