Determined DNA sequences derived from a papillomavirus genome, their uses for in vitro diagnostic purposes and the production of antigenic compositions

• US 6,107,086 A
• Filed: 06/11/1999
• Issued: 08/22/2000
• Est. Priority Date: 03/21/1986
• Status: Expired due to Fees

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Institut Pasteur
• Inventors: Cole, Stewart, Streeck, Rolf E.
• Primary Examiner(s): SALIMI, ALI REZA
• Application Number:
  US09/330,076
• Time in Patent Office: 438 Days
• Field of Search: 435/320.1, 435/172.3, 435/5, 536/235.1, 536/23.72, 536/23.1, 536/24.1, 514/44
• US Class Current: 435/320.1
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2710/20022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: C12Q 1/708 for papilloma

Abstract

The invention concerns DNA fragments derived from the genomic DNA of HPV-33. These fragments are selected from the group of fragments extending between the nucleotide extremities defined hereafter in relation to the nucleotide-numbering in FIGS. 1a and 1b respectively:

76-556

543-864

867-2811

2728-3808

3326-3575

3842-4079

4198-5611

5516-8091.

The invention also relates to the use of these fragments as probes for the detection of HPV in tissue cultures.

Claims

1. A purified nucleotide sequence selected from the group consisting of (a) a DNA consisting of the nucleotide sequence as shown in FIGS. 1A-1B and (b) a DNA fully complementary to sequence (a).

4. A recombinant host cell comprising the nucleotide sequence of claim 1.

2. A purified nucleotide sequence consisting of the nucleotide sequence as shown in FIGS. 1A-1B.

3. A vector comprising a nucleotide sequence according to claim 2.

5. A recombinant host cell comprising the nucleotide sequence of claim 2.

6. A recombinant host cell comprising the vector according to claim 3.