Isolated porcine respiratory and reproductive virus, vaccines and methods of protecting a pig against a disease caused by a porcine respiratory and reproductive virus

• US 6,110,467 A
• Filed: 05/13/1997
• Issued: 08/29/2000
• Est. Priority Date: 10/30/1992
• Status: Expired due to Term

• Current Assignee: Zoetis WHC 2 LLC (Zoetis Incorporated)
• Original Assignee: Iowa State University Research Foundation Incorporated (Iowa State University), Solvay Animal Health, Inc. (Pfizer Inc.)
• Inventors: Meng, Xiang-Jin, Lum, Melissa Anne, Halbur, Patrick G., Paul, Prem S., Lyoo, Young S.
• Primary Examiner(s): Caputa, Anthony C.
• Application Number:
  US08/855,531
• Time in Patent Office: 1,204 Days
• Field of Search: 435/235.1, 435/236, 435/237, 435/238, 435/239, 424/204.1, 424/209.1, 424/218.1, 424/815
• US Class Current: 424/204.1
• CPC Class Codes: A61K 2039/5254 avirulent or attenuated
• CPC Class Codes: A61K 2039/543 intranasal
• CPC Class Codes: A61K 2039/552 Veterinary vaccine
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61P 15/00 Drugs for genital or sexual disorders for disorders of sex hormones A61P5/24; Contraceptives
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2770/10021 Viruses as such, e.g. new isolates, mutants or their genomic sequences
• CPC Class Codes: C12N 2770/10022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2770/10034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: G01N 33/56983 Viruses
• CPC Class Codes: Y10S 424/815 Viral vaccine for porcine species, e.g. swine

Abstract

The present invention provides a vaccine which protects pigs from a virus and/or an infectious agent causing a porcine respiratory and reproductive disease, a method of protecting a pig from a disease caused by a virus and/or an infectious agent which causes a respiratory and reproductive disease, a method of producing a vaccine against a virus and/or an infectious agent causing a porcine reproductive and respiratory disease, and a biologically pure sample of a virus and/or infectious agent associated with a porcine respiratory and reproductive disease, particularly the Iowa strain of porcine reproductive and respiratory syndrome virus (PRRSV), and an isolated polynucleotide which is at least 90% homologous with a polynucleotide obtained from the genome of a virus and/or infectious agent which causes a porcine respiratory and reproductive disease.

Claims

1. A naturally occurring isolated virus selected from the group consisting of ISU-51 (VR 2428), ISU-55 (VR 2430), ISU-3927 (VR 2431) and ISU-1894 (VR 2475).

2. A composition comprising the isolated virus of claim 1 and a physiologically acceptable carrier.

3. A vaccine which protects a pig against porcine reproductive and respiratory syndrome (PRRS), comprising an inactivated or attenuated virus prepared by serial passage in cell culture and a physiologically acceptable carrier, wherein prior to inactivation or attenuation, said virus is the virus of claim 1.

4. The vaccine of claim 3, wherein lung lesions in said pig are reduced by a statistically significant amount, wherein said amount is significant at a p value less than 0.01, relative to lung lesions in uninoculated pigs.

5. The vaccine of claim 3 comprising an effective amount of said inactivated or attenuated virus which lowers the average clinical respiratory score of a group of pigs inoculated with said vaccine, then subsequently challenged with live PRRS virus, relative to a group of identically challenged pigs not inoculated with the vaccine.

6. The vaccine of claim 3 further comprising an adjuvant.

7. A method of protecting a pig from a porcine reproductive and respiratory disease, comprising administering an effective amount of the vaccine of claim 3 to a pig in need of protection against said disease.

8. The method of claim 7, wherein said vaccine is administered orally or parenterally.

9. The method of claim 7, wherein said vaccine is administered intramuscularly, intradermally, intravenously, intraperitoneally, subcutaneously or intranasally.

10. The method of claim 7, wherein said vaccine is administered to a sow in need of protection against said disease.