Procedure for quantitative and qualitative determination of chemical substances, based on molecular recognition and measurement of magnetic permeability
First Claim
1. A system for in vitro detection of an analyte, said system comprising a marker, a recognition element, and a transducer, and a vessel, said marker comprising a material having an externally, inductively detectable relative magnetic permeability constant of at least about 600, said recognition element being capable of binding to or competing for binding with said analyte in said vessel, and said transducer being adapted to be induced proportional to a magnetic permeability of a sample, wherein said transducer comprises a coil, and wherein said vessel containing said sample is introduced into said coil, wherein said marker and said recognition element act to induce in said transducer a first inductance value when said analyte is absent from said sample, and a second inductance value when said analyte is present in said sample.
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Abstract
A system for very sensitive detection and/or quantification of an analyte is disclosed. The system is based on an interaction, such as specific binding or competitive displacement from a ligand, between an analyte and a ferromagnetic marker. The great difference in magnetic permeability between the ferromagnetic marker and the background allows detection of very small quantities of analyte, based on the marker'"'"'s induction of a changed magnetic permeability for the sample being measured.
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Citations
58 Claims
- 1. A system for in vitro detection of an analyte, said system comprising a marker, a recognition element, and a transducer, and a vessel, said marker comprising a material having an externally, inductively detectable relative magnetic permeability constant of at least about 600, said recognition element being capable of binding to or competing for binding with said analyte in said vessel, and said transducer being adapted to be induced proportional to a magnetic permeability of a sample, wherein said transducer comprises a coil, and wherein said vessel containing said sample is introduced into said coil, wherein said marker and said recognition element act to induce in said transducer a first inductance value when said analyte is absent from said sample, and a second inductance value when said analyte is present in said sample.
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29. A method of detecting an analyte, comprising the steps of:
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providing a sample, said sample comprising a marker and a recognition element, said marker comprising a material having an externally, inductively detectable relative magnetic permeability constant of at least about 600, said recognition element being capable of binding to or competing for binding with said analyte, said marker and said recognition element being adapted to cooperate to induce in a transducer comprising a coil a first inductance value when said analyte is absent from said sample, and a second inductance value when said analyte is present in said sample; inducing an inductance value in said transducer by positioning said sample in said coil of said transducer in vitro; and determining whether said analyte is absent or present in said sample, based on said inductance value induced in said inducing step. - View Dependent Claims (30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57)
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58. A method for detecting the presence of an analyte, comprising the steps of:
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contacting, in vitro, a sample suspected of containing said analyte with a reagent that binds to or competes for binding with or by said analyte, said reagent including a component having an externally, inductively detectable relative magnetic permeability constant of at least about 600; separating unbound reagent from bound reagent; and detecting or measuring the bound or unbound reagent as an indication of the presence, absence, or quantity of said analyte in said sample by placing said sample in a coil of a transducer adapted to be induced proportional to a magnetic permeability of said sample.
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Specification