System for simultaneously conducting multiple ligand binding assays

• US 6,110,749 A
• Filed: 09/04/1997
• Issued: 08/29/2000
• Est. Priority Date: 03/01/1996
• Status: Expired due to Fees

• Current Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Original Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Inventors: Silzel, John W., Obremski, Robert J.
• Primary Examiner(s): Swartz, Rodney P.
• Application Number:
  US08/923,786
• Time in Patent Office: 1,090 Days
• Field of Search: 356/317, 356/318, 356/244, 356/246, 385/12, 385/13, 385/144, 422/57, 422/82.05, 422/82.08, 422/82.11, 435/4, 436/501, 436/518, 436/527
• US Class Current: 436/527
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A system for simultaneously conducting multiple ligand assays on a sample potentially containing target analytes uses as a detector a waveguide having a planar surface with a plurality of probes of known recognition to the target analytes thereon. The probes are in discrete areas on the waveguide. A sample containing target analyte is treated with a light-responsive compound such that it binds to the target analyte to form a conjugate and the conjugate is applied to the probes on the waveguide. A laser light is passed into the planar surface of the waveguide at a plurality of different locations, by causing relative movement between the waveguide and the laser light, so that evanescent waves radiate from the waveguide. Where conjugate has attached to a probe, there is emission of light different from that emitted by a probe without conjugate attached thereto.

Claims

1. A method for detecting a target analyte in a sample, the method comprising the steps of:

a) providing a detector comprising a waveguide having opposed planar surfaces with a plurality of discrete probes on at least one of the planar surfaces, each probe including a specific binding partner for a selected analyte, at least one of the probes being a responsive probe that includes a specific binding partner for the target analyte;

b) applying the sample to the detector such that the target analyte binds to its specific binding partner;

c) passing laser light into a first selected location of one of the planar surfaces of the detector so that evanescent light radiates from the waveguide and impinges on at least some of the probes, wherein light, if any, emitted from a probe with target analyte bound thereto is different from the light, if any, emitted by the same probe without target analyte bound thereto;

d) detecting emission of light from at least one first selected probe;

e) causing relative movement between the laser light and the detector;

f) passing laser light into a second selected location of one of the planar surfaces of the detector, so that evanescent light radiates from the waveguide and impinges on at least some of the probes, wherein the second selected location is different from the first selected location, wherein light, if any, emitted from a probe with a target analyte bound thereto is different from the light, if any, emitted by the same probe without target analyte bound thereto; and

g) detecting emission of light from at least one second selected probe which is different from the first selected probe.

11. The method of claim 1 wherein the step of causing relative movement comprises moving the waveguide relative to the laser light while the laser light is maintained in fixed position.

2. A method for detecting a target analyte in a sample, the method comprising the steps of:

a) providing a detector comprising a waveguide having a planar surface with a plurality of discrete probes on the planar surface, each probe including a specific binding partner for a selected analyte, at least one of the probes being a responsive probe that includes a specific binding partner for the target analyte;

b) applying the sample to the detector such that the target analyte binds to a specific binding partner;

c) passing laser light into the detector at a plurality of selected locations on the planar surface by causing relative movement between the waveguide and the laser light so that evanescent light radiates from the waveguide and impinges on at least some of the probes, wherein light, if any, emitted from a probe with target analyte bound thereto is different from the light, if any, emitted by the same probe without target analyte bound thereto; and

d) detecting emission of light from the probes.

3. The method of claim 2 wherein a probe without target analyte bound thereto emits substantially no light and a probe with target analyte bound thereto emits sufficient light to be detected during the step of detecting.

4. The method of claim 3 comprising the step of attaching to the target analyte, before or after the target analyte binds to its specific binding partner, a light-emitting compound, wherein the light-emitting compound emits light when the laser light is passed into the detector.

5. The method of claim 4 wherein the light-emitting compound is attached to the target analyte before the sample is applied to the detector.

6. The method of claim 2 wherein each probe includes a light-emitting compound.

7. The method of claim 2 wherein the probes include a light-emitting compound, and the method comprises the step of attaching to the target analyte, before or after the target analyte binds to its specific binding partner, a light-modulating compound, wherein the light-modulating compound affects the light emitted by the light-emitting compound when the laser light is passed into the detector.

8. The method of claim 7 wherein the light-modulating compound is a quencher that prevents the light-emitting compound from emitting light.

9. The method of claim 7 wherein the light-modulating compound is attached to the target analyte before the sample is applied to the detector.

10. The method of claim 2 including the additional step of removing a substantial portion of the sample from the detector before passing laser light into the detector.

21. The method of claim 4 wherein the light-emitting compound is a fluorophore.

22. The method of claim 2 wherein the laser light has a wavelength of from about 600 to about 960 nanometers.

23. The method of claim 2 wherein the specific binding partners are covalently bonded to the waveguide.

12. A method for detecting a target analyte in a sample, the method comprising the steps of:

a) providing a detector comprising a waveguide having a planar surface thereon and having a plurality of discrete probes, each probe including a specific binding partner for a selected analyte, a plurality of the probes being a responsive probe that includes a specific binding partner for the target analyte;

b) exposing the probes to (i) the sample and (ii) an analog of the target analyte, the analog being capable of binding to the same specific binding partner to which the target analyte can bind, so that the analog and the target analyte competitively bind to the responsive probes;

c) passing laser light into the detector at a plurality of selected locations on the planar surface by causing relative movement between the waveguide and the laser light so that evanescent light radiates from the waveguide and impinges on the probes, wherein light, if any, emitted from a probe with target analyte bound thereto is different from the light, if any, emitted by the same probe with the analog bound thereto; and

d) detecting emission of light from the probes.

13. The method of claim 12 wherein the analogs include a light-emitting compound so that only responsive probes with analog bound thereto emit light.

14. The method of claim 12 including the additional step of removing a substantial portion of the sample from the detector before passing laser light into the detector.

15. A method for analyzing multiple samples for the same target analyte, the method comprising the steps of:

a) providing a detector comprising a waveguide having a planar surface with a plurality of discrete probes thereon, at least some of the probes being a responsive probe that includes a specific binding partner for the target analyte;

b) treating each sample with a different light-emitting compound so that the light-emitting compounds attach to the target analyte in the samples to form conjugates;

c) applying the samples to the detector such that the conjugates bind to the responsive probes;

d) passing laser light into the detector at a plurality of selected locations on the planar surface by causing relative movement between the waveguide and the laser light so that evanescent light radiates from the waveguide and impinges on the responsive probes resulting in light being emitted by the light-emitting compounds, wherein the different light-emitting compounds emit different light; and

e) detecting emission of light from the probes.

16. The method of claim 15 wherein the different light-emitting compounds emit light that differs in the wavelength, intensity or color.

17. A method for detecting multiple target analytes in a sample, the method comprising the steps of:

a) providing a detector comprising a waveguide having a planar surface with a plurality of different discrete probes thereon, each probe including a specific binding partner for a selected analyte, at least some of the probes being capable of binding to a corresponding target analyte;

b) applying the sample to the detector such that the target analytes bind to their corresponding specific binding partner;

c) passing laser light into the detector at a plurality of selected locations on the planar surface by causing relative movement between the waveguide and the laser light so that evanescent light radiates from the waveguide and impinges on the probes, wherein light, if any, emitted from a probe with target analyte bound thereto is different from the light, if any, emitted by the same probe without target analyte bound thereto; and

d) detecting emission of light from the probes.

18. The method of claim 17 wherein a probe without target analyte bound thereto emits substantially no light and a probe with target analyte bound thereto emits sufficient light to be detected during the step of detecting.

19. The method of claim 17 comprising the step of treating the target analytes with a plurality of different light-emitting compounds, each light emitting compound attaching to a corresponding target analyte, wherein the light-emitting compounds emit light when the laser light is passed into the detector, the different light-emitting compounds emitting different light.

20. The method of claim 17 wherein the light-emitting compounds are attached to the target analyte before the sample is applied to the detector.