Lactoferrin variants and uses thereof
First Claim
1. A nucleic acid sequence encoding a lactoferrin variant or portion thereof, wherein said portion is further defined to comprise an amino acid sequence corresponding to at least one iron binding site of lactoferrin, and wherein the lactoferrin variant or portion thereof has a modified iron binding capacity, and wherein the amino acid sequence corresponding to at least one iron binding site of lactoferrin comprises a mutation or deletion of one or more amino acids selected from the group consisting of Asp 396, Tyr 93. Tyr 193 and His 254 in the amino-terminal lobe and Asp 396. Tyr 436. Tyr 529 and His 598 in the carboxy-terminal lobe.
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Accused Products
Abstract
The present invention is directed to recombinant nucleic acids encoding lactoferrin variants and portions thereof, having modified iron-binding capacity, and to vectors comprising same recombinant nucleic acids. The present invention is further directed to methods of producing such vectors, and to transfected cells harboring the same. Methods for the production of lactoferrin variants and portions thereof, in various eukaryotic or prokaryotic cells are also provided. Finally, the invention is directed to lactoferrin variants and portions thereof encoded by the nucleic acids of the invention and produced by the processes of the invention. Thus, the invention provides an efficient and economical means for the production of recombinant lactoferrin variants and portions thereof.
54 Citations
15 Claims
- 1. A nucleic acid sequence encoding a lactoferrin variant or portion thereof, wherein said portion is further defined to comprise an amino acid sequence corresponding to at least one iron binding site of lactoferrin, and wherein the lactoferrin variant or portion thereof has a modified iron binding capacity, and wherein the amino acid sequence corresponding to at least one iron binding site of lactoferrin comprises a mutation or deletion of one or more amino acids selected from the group consisting of Asp 396, Tyr 93. Tyr 193 and His 254 in the amino-terminal lobe and Asp 396. Tyr 436. Tyr 529 and His 598 in the carboxy-terminal lobe.
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2. A nucleic acid sequence encoding a lactoferrin variant selected from the group consisting of MN-2Y, MC-2Y and MNC-4Y.
- 6. A vector selected from the group consisting of p26MN-2Y, p26MC-2Y and p26MNC-4Y.
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15. A lactoferrin variant selected from the group consisting of MN-2Y, MC-2Y and MNC-4Y.
Specification