Dibenzorhodamine dyes
First Claim
1. A labelled nucleoside or nucleotide compound comprising a dibenzorhodamine dye of the structure:
- ##STR21## including nitrogen- and aryl-substituted forms thereof; and
a nucleoside, nucleotide or analog thereof,wherein the dye and the nucleoside, nucleotide or analog are connected by a linkage, the linkage is attached to the 8-position of the purine, if the nucleoside, nucleotide or analog comprises a 7-deazapurine base, the linkage is attached to the 7-position of the 7-deazapurine, and if the nucleoside, nucleotide or analog comprises a pyrimidine base, the linkage is attached to the 5-position of the pyrimidine.
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Accused Products
Abstract
Dibenzorhodamine compounds having the structure ##STR1## are disclosed, including nitogen- and aryl-substituted forms thereof. In addition, two intermediates useful for synthesizing such compounds are disclosed, a first intermediate having the structure ##STR2## including nitrogen- and aryl-substituted forms thereof, and a second intermediate having the structure ##STR3## including nitrogen- and aryl-substituted forms thereof, wherein substituents at positions C-14 to C18 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, --CH2 OH, alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further includes energy transfer dyes comprising the dibenzorhodamine compounds, nucleosides labeled with the dibenzorhodarnine compounds, and nucleic acid analysis methods employing the dibenzorhodamine compounds.
54 Citations
44 Claims
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1. A labelled nucleoside or nucleotide compound comprising a dibenzorhodamine dye of the structure:
- ##STR21## including nitrogen- and aryl-substituted forms thereof; and
a nucleoside, nucleotide or analog thereof,wherein the dye and the nucleoside, nucleotide or analog are connected by a linkage, the linkage is attached to the 8-position of the purine, if the nucleoside, nucleotide or analog comprises a 7-deazapurine base, the linkage is attached to the 7-position of the 7-deazapurine, and if the nucleoside, nucleotide or analog comprises a pyrimidine base, the linkage is attached to the 5-position of the pyrimidine. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
- ##STR21## including nitrogen- and aryl-substituted forms thereof; and
Specification