Please download the dossier by clicking on the dossier button x
×

Thiol-derivatized oligonucleotides

  • US 6,114,513 A
  • Filed: 09/05/1997
  • Issued: 09/05/2000
  • Est. Priority Date: 01/11/1990
  • Status: Expired due to Term
First Claim
Patent Images

1. A method comprising the step of contacting a biological sample suspected of containing an RNA with a compound comprising a plurality of linked nucleosides, wherein:

  • each nucleoside comprises a ribofuranosyl sugar portion and a base portion; and

    at least one of said nucleosides bears at a 2'"'"'-O-position, a 3'"'"'-O-position, or a 5'"'"'-O-position a substituent having formula;

    
    
    space="preserve" listing-type="equation">--R.sub.S --S--R.sub.1where;

    RS has formula RA, RA --C(O)--Q--RA, RA --Q--RA --Q--RA ;

    each RA is independently selected from alkyl having from 1 to about 10 carbon atoms;

    each Q is independently selected from the group of NH, O, and S;

    R1 is H or a thiol protecting group or has formula S--R2, CH2 C(O)--NH--R2, CH2 --CH═

    CH--C(O)--R2, --CH2 --CH2 --NH--S(O)2 --R2, or (maleimido)-R2 ; and

    R2 comprises a steroid molecule, a reporter molecule, a lipophilic molecule, a reporter enzyme, a peptide, a protein, a reporter group, an alkylator, an intercalator, a cell receptor binding molecule, a crown ether, a crown amine, a porphyrin, a crosslinking agent, a peptide nucleic acid, or a thiol attached to a poly(ethylene glycol).

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×