Cholesterol sensor and method for producing the same
First Claim
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1. A cholesterol sensor comprising:
- an electrically insulating base plate;
an electrode system composed of at least a measuring electrode and a counter electrode and disposed on said base plate, anda dry reaction layer formed on said electrode system,wherein said reaction layer contains cholesterol oxidase, cholesterol esterase and a surfactant,wherein said reaction layer is water soluble,wherein said electrode system comprises a silver coating and a carbon coating on said silver coating, andwherein said reaction layer comprises at least two layers, a surfactant-free layer formed on said electrode system and a layer containing a surfactant.
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Abstract
A cholesterol sensor facilitating rapid measurement of serum cholesterol levels is disclosed. The sensor comprises an electrode system composed of at least a measuring electrode and a counter electrode and disposed on an electrically insulating base plate and a reaction layer formed on or in the vicinity of the electrode system. The reaction layer contains cholesterol esterase for catalyzing the conversion of cholesterol ester into cholesterol, cholesterol oxidase and a surfactant.
46 Citations
25 Claims
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1. A cholesterol sensor comprising:
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an electrically insulating base plate; an electrode system composed of at least a measuring electrode and a counter electrode and disposed on said base plate, and a dry reaction layer formed on said electrode system, wherein said reaction layer contains cholesterol oxidase, cholesterol esterase and a surfactant, wherein said reaction layer is water soluble, wherein said electrode system comprises a silver coating and a carbon coating on said silver coating, and wherein said reaction layer comprises at least two layers, a surfactant-free layer formed on said electrode system and a layer containing a surfactant. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A cholesterol sensor comprising:
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an electrically insulating base plate, an electrode system composed of at least a measuring electrode and a counter electrode and disposed on said base plate, and a dry reaction layer formed on or in the vicinity of said electrode system, wherein said reaction layer contains cholesterol oxidase, cholesterol esterase and a surfactant, wherein said reaction layer is water soluble, wherein said electrode system comprises a silver coating and a carbon coating on said silver coating, wherein a cover member is provided to form a sample solution supply pathway for supplying a sample solution to said electrode system between said base plate and said cover member by integrating it with said base plate, two hydrophilic polymer layers are formed, a first hydrophilic polymer layer is formed on a surface exposed to the sample solution supply pathway formed by said cover member and a second hydrophilic polymer layer is formed on said electrode system, and said reaction layer is split into a first segment and a second segment and formed, respectively, on said two hydrophilic polymer layers, wherein said first segment formed on said first hydrophilic polymer layer contains a surfactant and said second segment formed on said second hydrophilic polymer layer does not contain a surfactant.
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9. A cholesterol sensor comprising:
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an electrically insulating base plate, an electrode system composed of at least a measuring electrode and a counter electrode and disposed on said base plate, and a dry reaction layer formed on or in the vicinity of said electrode system, wherein said reaction layer contains cholesterol oxidase, cholesterol esterase and a surfactant, wherein said electrode system comprises silver and carbon for coating said silver, wherein said reaction layer comprises two layers, a layer containing cholesterol oxidase and cholesterol esterase only omitting a surfactant and a layer containing a surfactant and covering the former layer. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
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17. A cholesterol sensor comprising:
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an electrically insulating base plate, an electrode system composed of at least a measuring electrode and a counter electrode and disposed on said base plate, and a dry reaction layer formed on or in the vicinity of said electrode system, wherein said reaction layer contains cholesterol oxidase, cholesterol esterase and a surfactant, wherein said reaction layer comprises a layer of cholesterol oxidase and cholesterol esterase omitting a surfactant and a layer containing a surfactant and covering the former layer, wherein said electrode system comprises a silver coating and a carbon coating on said silver coating, and wherein said reaction layer is water soluble. - View Dependent Claims (18, 19, 20, 21, 22, 23)
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24. A method for producing a cholesterol sensor comprising the steps of:
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forming an electrode system by printing a silver paste on an electrically insulating base plate and further printing a carbon paste thereon to cover said silver paste, forming a hydrophilic polymer layer on said electrode system by dropping thereon a solution containing a hydrophilic polymer, forming an enzyme layer by adding aliquots of a solution containing at least cholesterol oxidase and cholesterol esterase but no surfactant over said hydrophilic polymer layer, followed by drying, and forming a surfactant layer on said enzyme layer, wherein said surfactant layer is formed by adding aliquots of a solution prepared by dissolving a surfactant in a solvent that would not dissolve said enzyme layer, followed by drying.
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25. A cholesterol sensor comprising:
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an electrically insulating base plate, an electrode system composed of at least a measuring electrode and a counter electrode and disposed on said base plate, and a dry reaction layer formed on or in the vicinity of said electrode system, wherein said reaction layer contains cholesterol oxidase, cholesterol esterase and a surfactant, wherein said reaction layer is water soluble, wherein said electrode system comprises a silver coating and a carbon coating on said silver coating, wherein a cover member is provided to form a sample solution supply pathway for supplying a sample solution to said electrode system between said base plate and said cover member by integrating it with said base plate, and wherein said reaction layer comprises at least two layers, a surfactant-free layer formed on said electrode system and a layer containing a surfactant.
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Specification