System for releasing and isolating nucleic acids

• US 6,117,398 A
• Filed: 02/17/1998
• Issued: 09/12/2000
• Est. Priority Date: 04/01/1995
• Status: Expired due to Fees

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Inventors: Schubert, Ulrich, Kolb, Uwe, Bienhaus, Gerhard, Pasch, Manfred, Stolz, Burkhard
• Primary Examiner(s): LUDLOW, JAN M
• Application Number:
  US08/930,247
• Time in Patent Office: 938 Days
• Field of Search: 422/99-104, 436/526, 435/6, 435/286.7, 209/215, 209/217, 209/219, 209/225, 210/695, 210/222, 210/223, 536/25.4, 536/25.41
• US Class Current: 422/527
• CPC Class Codes: C12N 15/1013 by using magnetic beads
• CPC Class Codes: C12Q 1/6804 Nucleic acid analysis using immunogens immunoassay G01N33/53
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C

Abstract

An instrument that can hold one or more sample processsing vessels, maintain the sample processing vessels at a constant temperature, shake the sample processing vessels and separate magnetic particles by means of magnetic force. This system greatly simplifies the isolation of nucleic acids.

Claims

1. A system, comprising

a sample processing vessel having an outlet opening and being capable of containing fluid and biological compartments;

a receptacle defining at least one cavity which holds therein the sample processing vessel, wherein said receptacle comprises a hose connection for connecting a hose to the outlet opening;

a plurality of magnetic particles for introducing into the sample processing vessel, wherein said magnetic particles are capable of binding with the biological compartments;

a thermostat unit configured to maintain the sample processing vessel and its contents at an essentially constant temperature;

a mechanical shaker configured to shake the sample processing vessel; and

a magnetic means for magnetically attracting said magnetic particles to an inner wall of the sample processing vessel,

wherein said receptacle, said thermostat unit and said magnetic means are integrated into a reaction block.

2. The system of claim 1, wherein said sample processing vessel contains biological compartments.

3. The system of claim 1, further comprising a hose which is connected to said hose connection.

4. The system of claim 1, further comprising a hose, wherein one end of said hose is connected to said hose connection.

5. The system of claim 1, wherein said magnetic means and said receptacle are configured to move towards or away from one another.

6. The system of claim 1, wherein said receptacle defines at least two cavities, wherein each cavity is configured to hold therein a sample processing vessel.

7. The system of claim 6, further comprising another sample processing vessel, wherein each of the sample processing vessels is held in a corresponding cavity.

8. The system of claim 6, wherein the cavities are arranged in an essentially linear format.

9. The system of claim 1, wherein said mechanical shaker comprises an eccentric drive.

10. The system of claim 1, wherein said thermostat unit comprises a thermally conductive metal block which is integrated into said receptacle, and the at least one cavity is configured to snugly hold therein the sample processing vessel such that said metal block is capable of efficiently transferring heat between said metal block and the sample processing vessel.

11. The system of claim 1, wherein said thermostat unit comprises a resistance heating unit and a peltier element.

12. The system of claim 1, wherein said sample processing vessel and the at least one cavity have essentially matching conicity.

13. The system of claim 1, wherein said thermostat unit regulates the temperature by means of a fuzzy regulator.

14. The system of claim 1, wherein said thermostat unit regulates the temperature by means of a proportion integral differential time regulator.

15. The system of claim 1, wherein said thermostat unit comprises a block through which a thermostating medium flows.

16. The system of claim 1, wherein said magnetic particles comprise ferromagnetic particles.

17. The system of claim 1, further comprising a pump unit which is connected to said hose connection, wherein said pump unit is configured to remove the fluid from the outlet opening through the hose.

18. A system, comprising

a sample processing vessel having an outlet opening and being capable of containing fluid and biological compartments;

a receptacle defining at least one cavity which holds therein the sample processing vessel, wherein said receptacle comprises a hose connection for connecting a hose to the outlet opening;

a plurality of magnetic particles for introducing into the sample processing vessel, wherein the magnetic particles are capable of binding with the biological compartments;

a thermostat means for maintaining the sample processing vessel and its contents at an essentially constant temperature;

a shaking means for shaking the sample processing vessel; and

a magnetic means for magnetically attracting said magnetic particles to an inner wall of the sample processing vessel,

wherein said receptacle, said thermostat means and said magnetic means are integrated into a reaction block.

19. The system of claim 18, further comprising a pump unit which is connected to said hose connection, wherein said pump unit is configured to remove the fluid from the outlet opening through the hose.