Molecular detection apparatus and method

• US 6,117,630 A
• Filed: 09/27/1999
• Issued: 09/12/2000
• Est. Priority Date: 10/30/1997
• Status: Expired due to Fees

• Current Assignee: William Reber LLC
• Original Assignee: Motorola, Inc. (Motorola Solutions, Inc.)
• Inventors: Reber, William L., Toler, Jeffrey G., Heng, John E.
• Primary Examiner(s): Leary, Louise N.
• Application Number:
  US09/405,959
• Time in Patent Office: 351 Days
• Field of Search: 435/4, 435/283.1, 435/288.5, 435/287.2, 422/72
• US Class Current: 435/4
• CPC Class Codes: B01J 2219/00536 in the shape of disks
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence

Abstract

An apparatus comprises a disk-shaped support member (20) supporting a first sample-processing device (28) and a second sample-processing device (30). The first sample-processing device (28) has an elongate portion oriented along a first radial axis (32) of the support member (20). The second sample-processing device (30) has an elongate portion oriented along a second radial axis (34) transverse to the first radial axis (32). A sample-processing device can comprise a plurality of conduits (150) interconnected in a tree topology to couple an inlet (152) with a plurality of outlets (154). The inlet (152) is located at a root vertex and each of the outlets (154) is located at a corresponding leaf vertex of the tree topology. Each of a plurality of molecular detection chambers (156) is coupled to a respective one of the outlets (154). A method of using the apparatus is disclosed.

Claims

1. A molecular detection apparatus comprising:

a disk-shaped support member;

a first sample-processing device supported by the disk-shaped support member, the first sample-processing device having an elongate portion oriented along a first radial axis of the disk-shaped support member;

a second sample-processing device supported by the disk-shaped support member, the second sample-processing device having an elongate portion oriented along a second radial axis of the disk-shaped support member, wherein the second radial axis is transverse to the first radial axis; and

a cover mountable to the disk-shaped support member to enclose the first sample-processing device and the second sample-processing device.

2. The molecular detection apparatus of claim 1 wherein the cover is transparent.

3. A molecular sensing method comprising the steps of:

providing a first region having a first plurality of molecular probes;

providing a second region in communication with the first region;

applying a plurality of molecules, a second plurality of molecular probes, and ligase to the first region;

communicating at least one unligated probe of the second plurality of molecular probes from the first region to the second region;

sensing at the first region for at least one of the first plurality of molecular probes ligated to at least one of the second plurality of molecular probes; and

sensing at the second region for the at least one unligated probe.

4. The molecular sensing method of claim 3 further comprising the steps of, prior to sensing at the second region:

dissociating at least one of the plurality of molecules from the first plurality of molecular probes; and

communicating the at least one of the plurality of molecules to the second region.

5. The molecular sensing method of claim 3 further comprising the steps of:

binding at least one of the plurality of molecules to at least one of the first plurality of molecular probes;

binding at least one of the second plurality of molecular probes to the at least one of the plurality of molecules; and

ligating the at least one of the second plurality of molecular probes to the at least one of the first plurality of molecular probes.

6. The molecular sensing method of claim 3 wherein the first plurality of molecular probes are immobilized at the first region.

7. The molecular sensing method of claim 3 wherein the second plurality of molecular probes are tagged.