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Pressure-enhanced extraction and purification

  • US 6,120,985 A
  • Filed: 05/22/1998
  • Issued: 09/19/2000
  • Est. Priority Date: 10/31/1997
  • Status: Expired due to Term
First Claim
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1. A method of lysing a cell, the method comprising:

  • providing a frozen cell under atmospheric pressure;

    while maintaining the cell at a subzero temperature, exposing the cell to an elevated pressure in a pressure chamber, the elevated pressure being sufficient to thaw the frozen cell at the subzero temperature;

    depressurizing the pressure chamber to freeze the cell at the subzero temperature; and

    repeating the exposing and depressurizing steps until the cell is lysed.

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