Isothermal transcription based assay for the detection and quantification of the chemokine rantes
First Claim
Patent Images
1. An oligonnuclotide selected from the group consisting of SEQ ID NO:
- 1-SEQ ID NO;
7 and SEQ ID NO;
8.
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Abstract
An isothermal transcription based amplification assay for the detection or quantification of chemokine RNA uses primers and probes for sequences within the gene for RANTES, MIP-lα and MIP-1 β. The quantitative system uses an internal control Q of a mutant version of each gene. Target specific primers and probes are also disclosed.
4 Citations
19 Claims
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1. An oligonnuclotide selected from the group consisting of SEQ ID NO:
- 1-SEQ ID NO;
7 and SEQ ID NO;
8.
- 1-SEQ ID NO;
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2. An oligonucleotide of about 15-26 nucleotides, comprising at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7 and SEQ ID NO;
8. - View Dependent Claims (10, 11, 12)
- 1, SEQ ID NO;
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3. A method for the detection or quantitation of RANTES RNA in a sample, comprising:
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a) obtaining a sample which may contain RANTES RNA; b) performing isothermal transcription based amplification on the sample with two oligonucleotide primers, a first primer which comprises at least 10 consecutive nucleotides of a sequence according to SEQ ID NO;
2, and a second primer which comprises at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO;
1 and SEQ ID NO;
5; andc) detecting or qantitating the resulting product of step b) whereby detection or quantitation of the amplification product indicates the presence or quantity of RANTES RNA in the sample. - View Dependent Claims (4, 5, 6, 13, 14, 15)
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7. A primer pair for the detection or quantitation of RANTES RNA in a sample, comprising one primer selected from the group consisting of SEQ ID NO:
- 1 and SEQ ID NO;
5, and one primer selected from the group consisting of SEQ ID NO;
2 and SEQ ID NO;
4. - View Dependent Claims (8, 9)
- 1 and SEQ ID NO;
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16. A pair of oligonucleotides for the detection or quantitation of RANTES RNA, a first oligonucleotide of said pair being about 15-26 nucleotides in length and comprising at least 10 consecutive nucleotides of a sequence according to SEQ ID NO:
- 2, and a second oligonucleotide of said pair being about 15-26 nucleotides in length and comprising at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO;
1 and SEQ ID NO;
5. - View Dependent Claims (17, 18, 19)
- 2, and a second oligonucleotide of said pair being about 15-26 nucleotides in length and comprising at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO;
Specification