Method for separation of liquid and solid phases for solid phase organic syntheses
First Claim
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1. A method for separating a liquid phase from a solid phase, comprising:
- (a) positioning an array of reaction vessels, said vessels containing a slurry of solid phase supports in a liquid, on the perimeter of a centrifuge rotor; and
(b) spinning the rotor of the centrifuge at a speed so that the solid phase supports sediment in a pocket of the vessels from which material cannot be removed by centrifugal force and the liquid in excess of the volume of the pocket is expelled from the top of the vessels while spinning.
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Abstract
A simple, efficient apparatus and method for separation of solid and liquid phases useful in methods of high-throughput combinatorial organic synthesis of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis. In a preferred embodiment, an apparatus and method of removal of liquid phase from solid phase compatible with microtiter plate type array(s) of reaction vessels is disclosed.
130 Citations
27 Claims
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1. A method for separating a liquid phase from a solid phase, comprising:
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(a) positioning an array of reaction vessels, said vessels containing a slurry of solid phase supports in a liquid, on the perimeter of a centrifuge rotor; and (b) spinning the rotor of the centrifuge at a speed so that the solid phase supports sediment in a pocket of the vessels from which material cannot be removed by centrifugal force and the liquid in excess of the volume of the pocket is expelled from the top of the vessels while spinning. - View Dependent Claims (2, 3, 4, 5, 9, 10, 11, 12, 13, 14, 15, 16)
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6. A method for separating a liquid phase from a solid phase, comprising:
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(a) positioning an array of reaction vessels, said vessels containing a slurry of solid phase supports in a liquid, on the perimeter of a centrifuge rotor in a tilted position; and (b) spinning the rotor of the centrifuge at a speed at which the centrifugal force on the radius corresponding to the vessels which are closest to the axis of rotation is substantially greater than the force of gravity, so that the solid phase supports sediment in a pocket of the vessels from which material cannot be removed by centrifugal force and the liquid in excess of the volume of the pocket is expelled from the top of the vessels while spinning. - View Dependent Claims (7, 8)
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17. A method of solid-phase synthesis of compounds, said method comprising:
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(a) providing a reaction vessel containing a first building block coupled to a solid support in a slurry of solid supports in a liquid; (b) positioning said vessel on the perimeter of a centrifuge rotor; (c) spinning said rotor at a speed sufficient to expel said liquid from the top of said vessel while spinning and deposit said slurry of solid supports in a pocket of the vessel from which material cannot be removed by centrifugal force, wherein the liquid expelled is that in excess of the volume of the pocket; and (d) adding a second building block to said reaction vessel. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification