Optical confocal device having a common light directing means

• US 6,121,603 A
• Filed: 12/01/1997
• Issued: 09/19/2000
• Est. Priority Date: 12/01/1997
• Status: Expired due to Fees

• Current Assignee: New Dimiension Research & Instrument Incorporated
• Original Assignee: New Dimiension Research & Instrument Incorporated
• Inventors: Webb, Robert H., Hang, Zhijiang, Lazarev, Victor
• Primary Examiner(s): LE, QUE TAN
• Application Number:
  US08/982,103
• Time in Patent Office: 1,023 Days
• Field of Search: 250/234, 250/235, 250/216, 250/208.1, 250/214.1, 250/201.3, 257/80-84, 372/21, 372/22, 372/70
• US Class Current: 250/216
• CPC Class Codes: G02B 21/0032 Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06
• CPC Class Codes: G02B 21/004 fixed arrays, e.g. switchable aperture arrays
• CPC Class Codes: G02B 21/0056 based on optical coherence, e.g. phase-contrast arrangements, interference arrangements
• CPC Class Codes: G02B 21/0072 details concerning resolution or correction, including general design of CSOM objectives
• CPC Class Codes: G02B 21/0076 arrangements using fluorescence or luminescence

Abstract

A confocal scanning imaging device for viewing an object, includes illumination means including an array of independently addressable microlasers, detection means including an array of detectors for detecting light from an object to which light from the illumination means has been directed. The array of microlasers and the array of detectors are combined into a single array, such as on a single chip, and in a pattern constructed and arranged so that different detectors are adjacent each microlaser so that light from an illuminated object may be directed back to the vicinity of the illuminating microlaser and be detected by the adjacent detector(s). There is optical means for directing light generated by the microlasers onto an object and for directing light from an object so illuminated onto detectors adjacent the illuminating microlaser. The optical means includes a bilens and a matching lens system including two objective lenses. The system is constructed so that the relationships are as follows:

Δ=δ.sub.1 f.sub.1 /f.sub.2

δ.sub.2 =δ.sub.1 f.sub.3 /f.sub.1

where Δ is the distance between the optical centers of the bilens, δ2 is the distance between sequentially illuminated points on the object, δ1 is the distance between neighboring elements in the array, the focal length of the bilens is f₂ and the focal length of the objective lens nearest the array is f₁ and the focal length of the objective lens furthest from the array is f₃.

Claims

1. In a confocal scanning device for viewing an object, of the type including illumination means including an array of independently addressable microlasers, optical means for directing light generated by the illumination means onto an object and detection means including the array of detectors for detecting light from the object to which light from the illumination means has been directed;

the improvement comprising;

a. said array of microlasers and said array of detectors being in a single array; and

b. said optical means directing light from the object onto said detection means.

2. In a confocal scanning device as defined in claim 1, where said array of microlasers and said array of detectors are on a single chip.

3. In a confocal scanning device as defined in claim 2, wherein there is at least one detector adjacent each microlaser and said optical means includes a bilens and an accompanying lens system.

4. In a confocal scanning device as defined in claim 3, wherein the accompanying lens system includes two objective lenses, and the relationship is as follows:

space="preserve" listing-type="equation">Δ

=δ

.sub.1 f.sub.1 /f.sub.2

space="preserve" listing-type="equation">δ

.sub.2 =δ

.sub.1 f.sub.3 /f.sub.1

where Δ

is the distance between the optical centers of the bilens, δ

2 is the distance between sequentially illuminated points on the object, δ

1 is the distance between neighboring elements in the array, the focal length of the bilens is f₂ and the focal length of the objective lens nearest the array is f₁ and the focal length of the objective lens furthest from the array is f₃.

5. In a confocal scanning device as defined in claim 2,

wherein the detectors are individually addressable.

6. In a confocal scanning device as defined in claim 5 wherein the detector and laser addressing is coordinated to provide information at the detectors significant of the object being scanned.

7. In a confocal scanning imaging device as defined in claim 5, further comprising imaging device means for forming the image of an object from the information detected by said detection means.

8. In a confocal scanning imaging device as defined in claim 4 wherein the device is a microscope.

9. In a confocal scanning imaging device as defined in claim 4 wherein the device is an ophthalmoscope.

10. A confocal scanning device for viewing an object, comprising:

a. illumination means including an array of independently addressable microlasers;

b. detection means including an array of independently addressable detectors for detecting light from the object to which light from the illumination means has been directed, said array of microlasers and said array of detectors being in a single array and in a pattern constructed and arranged so that different detectors are adjacent each microlaser so that light from an illuminated object may be directed back to the vicinity of the illuminating microlaser and be detected by the adjacent detectors; and

c. optical means for directing light generated by the microlasers onto the object and for directing light from the object so illuminated onto detectors adjacent the illuminating microlaser.

11. A confocal scanning device as defined in claim 10, further comprising means for controlling actuation of the independently addressable microlasers and detectors, wherein each microlaser has associated detectors, said actuation being in a predetermined sequence.

12. A confocal scanning device as defined in claim 11, wherein said means for controlling controls the microlasers and detectors to be actuated so that adjacent microlasers are actuated one after the other in a row.

13. A confocal scanning device as defined in claim 11, wherein said means for controlling controls the microlasers and detectors to be actuated so that nonadjacent microlasers are actuated one after the other.

14. A confocal scanning device as defined in claim 11, wherein said means for controlling controls the microlasers so that a plurality thereof and the associated detectors are actuated simultaneously, said simultaneously actuated microlasers being spaced from each other a sufficient distance that reflected and scattered light from one set of associated microlaser and detectors does not interfere with any other simultaneously actuated set of associated microlaser and detectors.

15. A confocal scanning device as defined in claim 10 where the optical system includes a bilens.

16. A confocal scanning device as defined in claim 15 wherein the device is an ophthalmoscope.

17. A confocal scanning device as defined in claim 15 wherein the device is a microscope.

18. A confocal scanning device as defined in claim 10 wherein the detector immediately adjacent the activated microlaser is activated at the same time.

19. A confocal scanning device as defined in claim 10, further comprising a bundle of fibers disposed between the microlaser/detector array and the object to provide a remote viewing of the object.

20. A confocal scanning imaging device as defined in claim 19 wherein the bundle of fibers is a coherent arrangement.

21. A confocal scanning imaging device as defined in claim 15 wherein the accompanying lens system includes two objective lenses, and the relationship is as follows:

space="preserve" listing-type="equation">Δ

=δ

.sub.1 f.sub.1 /f.sub.2

space="preserve" listing-type="equation">δ

.sub.2 =δ

.sub.1 f.sub.3 /f.sub.1

where Δ

is the distance between the optical centers of the bilens, δ

2 is the distance between sequentially illuminated points on the object, δ

1 is the distance between neighboring elements in the array, the focal length of the bilens is f₂ and the focal length of the objective lens nearest the array is f₁ and the focal length of the objective lens furthest from the array is f₃.

22. A confocal scanning imaging device for viewing an object, comprising:

a. illumination means including an array of independently addressable microlasers;

b. detection means including an array of independently addressable detectors for detecting light from an object to which light from the illumination means has been directed, said array of microlasers and said array of detectors being in a single combined array and in a pattern constructed and arranged so that different detectors are adjacent each microlaser so that light from an illuminated object may be transmitted back to the vicinity of the illuminating microlaser and be detected by the adjacent detectors;

c. optical means for directing light generated by the microlasers onto the object and for directing light from an object so illuminated onto detectors adjacent the illuminating microlaser; and

d. imaging device means for forming an image of the object from the information detected by said detection means.

23. A confocal scanning imaging device as defined in claim 22, further comprising a bundle of fibers is disposed between the microlaser/detector array and the object to provide a remote viewing of the object.

24. A confocal scanning imaging device as defined in claim 23 wherein the bundle of fibers is a non-coherent arrangement.

25. A confocal scanning imaging device as defined in claim 23,

wherein the bundle of fibers is a coherent arrangement.

26. A confocal scanning imaging device as defined in claim 22 wherein the device is arranged to operate in direct mode.

27. A confocal scanning imaging device as defined in claim 22 wherein the device is arranged to operate in indirect mode.

28. A confocal scanning imaging device as defined in claim 22 wherein the detectors immediately surrounding the activated microlaser are all activated for detection at the same time and the direct light transmitted from an object does not impinge upon any detector whereby the inactive mode is used.

29. A confocal scanning device as defined in claim 22 where the optical system includes a bilens.

30. A confocal scanning imaging device as defined in claim 29 wherein the accompanying lens system includes two objective lenses, and the relationship is as follows:

space="preserve" listing-type="equation">Δ

=δ

.sub.1 f.sub.1 /f.sub.2

space="preserve" listing-type="equation">δ

.sub.2 =δ

.sub.1 f.sub.3 /f.sub.1

where Δ

is the distance between the optical centers of the bilens, δ

2 is the distance between sequentially illuminated points on the object, δ

1 is the distance between neighboring elements in the array, the focal length of the bilens is f₂ and the focal length of the objective lens nearest the array is f₁ and the focal length of the objective lens furthest from the array is f₃.

31. An imaging device as defined in claim 22 further comprising a second combined array for producing two photon-induced excitations, said optical means combining the two-laser beams by a beam splitter or a mirror or lens, each combined array being two-dimensional detector arrays or charged-coupled devices (CCD) for detecting fluorescence, and said combined arrays including integrated circuits for modulating at femtoseconds range.

32. A confocal device as defined in claim 31 comprising means for assuring detection will be of fluorescence from the object.

33. A method for generating an image of an object, comprising the steps of:

a. generating time-variant electrical excitations,

b. addressing said electrical excitations to microlasers in an array of independently addressable microlasers to generate non-overlapping beams of coherent light,

c. directing said light onto the object, and

d. detecting, in an array of detectors, light resulting from any of light scattering, light reflection, or light transmission from the object by independently addressing each detector in the array when it is to detect reflected and remitted light,

e. the plane of said microlasers and the plane of said detecting being identical.

34. A method for generating an image of an object as defined in claim 33, wherein the microlaser array and the detector array are in a single array, and the spacing between lasers and detectors in said combined array is less than 90 μ

m.

35. A method for generating an image of an object as defined in claim 34, where the directing of light is accomplished by an optical system which includes a bilens and two objective lenses, and the relationship is as follows:

space="preserve" listing-type="equation">Δ

=δ

.sub.1 f.sub.1 /f.sub.2

space="preserve" listing-type="equation">δ

.sub.2 =δ

.sub.1 f.sub.3 /f.sub.1

where Δ

is the distance between the optical centers of the bilens, δ

2 is the distance between sequentially illuminated points on the object, δ

1 is the distance between neighboring elements in the array, the focal length of the bilens is f₂ and the focal length of the objective lens nearest the array is f₁ and the focal length of the objective lens furthest from the array is f₃.

36. A method for generating an image of an object as defined in claim 34, wherein the step of detecting includes the detecting of fluorescence from said object.