Nanoelectrode arrays

• US 6,123,819 A
• Filed: 03/19/1998
• Issued: 09/26/2000
• Est. Priority Date: 11/12/1997
• Status: Expired due to Fees

• Current Assignee: Protiveris, Inc.
• Original Assignee: Protiveris, Inc.
• Inventors: Peeters, John P.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/044,350
• Time in Patent Office: 922 Days
• Field of Search: 204/403, 204/400, 204/412, 204/280, 435/287.1, 422/68.1
• US Class Current: 204/452
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2565/607 being a sensor, e.g. electrode
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 977/792 Nucleic acid array, e.g. human genome array
• CPC Class Codes: Y10S 977/793 Protein array
• CPC Class Codes: Y10S 977/853 Biological sample
• CPC Class Codes: Y10S 977/874 Probe tip array

Abstract

An array of electrodes at the atomic or nano scale (nanoelectrodes) is built on a chip. The spatial distribution, height, width and electrochemical composition of the nanoelectrodes is varied, such that protein-specific electronic receptors are built directly on the chip with the nanoelectrodes without the use of any specific binding agents or molecules. Because of their size, a very large number of different receptors can be built as arrays on a single chip. The chip can be used to detect, characterize and quantify single molecules in solution such as individual proteins, complex protein mixtures, DNA or other molecules.

Claims

1. A sensor for detecting biological molecules, said sensor comprising:

a substrate;

an electrode on said substrate, said electrode having the capacity to bind a preselected biological molecule, said electrode extending from a principal surface of said substrate a distance of from 2 Angstroms to 5 nanometers and said electrode having a width of from 2 Angstroms to 5 nanometers.

2. The sensor recited in claim 1, wherein said substrate has a plurality of said electrodes.

3. The sensor recited in claim 2, wherein each of said electrodes has an identical chemical composition.

4. The sensor recited in claim 2, wherein at least one of said electrodes has a chemical composition which is different than another of said electrodes.

5. The sensor recited in claim 1, wherein said electrode has an outer coating.

6. The sensor recited in claim 2, wherein each of said electrodes has a chemical coating.

7. The sensor recited in claim 6, wherein each of said coatings has the same chemical composition.

8. The sensor recited in claim 6, wherein at least one of said coatings is different than another of said coatings.

9. The sensor recited in claim 2, wherein the plurality of said electrodes extend from a principal surface of said substrate and wherein at least one of said electrodes extends farther from said principal surface than another of said electrodes.

10. The sensor recited in claim 2, wherein the width of at least one of said electrode is greater than the width of another of said electrodes.

11. The sensor recited in claim 2, wherein said electrodes are spaced laterally from one another on said substrate.

12. The sensor recited in claim 2, wherein said electrodes are arranged in clusters on said substrate.

13. The sensor recited in claim 12, wherein said clusters are spaced to form an array.

14. The sensor recited in claim 2, wherein the electro-chemical properties, width and spacing of said electrodes complement and bind a site on said biological molecules.

15. The sensor recited in claim 2, wherein said electrodes are connected to nanowires.

16. The sensor recited in claim 2, further comprising a support structure for said substrate, said support structure being adapted to be received in a x-y fluorescent laser reader.

17. The sensor recited in claim 1, wherein said electrode is connected to at least one electrically conductive nanowire.

18. The sensor recited in claim 1, further comprising an interface connecting said sensor to a control system.

19. The sensor recited in claim 1, wherein said biological molecules are proteins.

20. A sensor for detecting proteins, said sensor comprising:

a micro-capillary tube;

a plurality of electrodes disposed in said tube, said electrodes having the capacity to bind a preselected protein, said electrodes being between about 10^-9 and 10^-10 meters in height and width.

21. The sensor recited in claim 20, further comprising a microcontroller.

22. The sensor recited in claim 21, further comprising a system to regulate the temperature of said sensor.

23. A method of sequencing nucleic acids, comprising the steps of:

providing a sensor assembly, said sensor assembly having a substrate on which plurality of electrodes are disposed, said electrodes each extending from a principal surface of said substrate a distance of from 2 Angstroms to 5 nanometers and having a width of from 2 Angstroms to 5 nanometers;

contacting said electrodes with a solution containing nucleic acids;

said electrodes having the capacity to bind at least some of said nucleic acids.

24. The method recited in claim 23, wherein said nucleic acids are DNA and wherein said electrodes are spaced apart from one another to complement and bind to DNA base pairs of a linear DNA molecule.

25. The method recited in claim 23, wherein said sensor assembly includes a microtube in which said electrodes are disposed.

26. The method recited in claim 25, wherein said sensor assembly further comprises a flow control system and a laser detector.

27. The method recited in claim 23, wherein said sensor assembly further comprises a microcontroller and a display.

28. The method recited in claim 23, wherein said nucleic acids are RNA.