Viral obesity methods and compositions

• US 6,127,113 A
• Filed: 04/06/1998
• Issued: 10/03/2000
• Est. Priority Date: 04/04/1997
• Status: Expired due to Term

• Current Assignee: Obetech LLC
• Original Assignee: Obetech LLC
• Inventors: Atkinson, Richard L., Dhurandhar, Nikhil V.
• Primary Examiner(s): Mosher, Mary E.
• Assistant Examiner(s): SALIMI, ALI REZA
• Application Number:
  US09/056,153
• Time in Patent Office: 911 Days
• Field of Search: 435/5, 435/6, 435/7.1, 424/204.1, 424/233.1, 424/235.1, 536/23.72, 530/328, 530/329
• US Class Current: 435/5
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61K 39/235 Adenoviridae
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 16/081 from DNA viruses
• CPC Class Codes: C07K 2317/76 Antagonist effect on antigen, e.g. neutralization or inhibition of binding
• CPC Class Codes: C12N 2710/10321 Viruses as such, e.g. new isolates, mutants or their genomic sequences
• CPC Class Codes: C12N 2710/10322 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2710/10334 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/701 Specific hybridization probes
• CPC Class Codes: G01N 2333/075 Adenoviridae
• CPC Class Codes: G01N 2800/044 Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity
• CPC Class Codes: G01N 33/56983 Viruses

Abstract

A source of viral induced obesity has been discovered. A virus known as AD-36P has been found to be associated with obesity in both animals and humans. Diagnostic DNA sequences are presented so that DNA based tests for the presence of the obesity associated virus can be conducted.

Claims

1. A method for testing a subject for viral induced obesity to determine if the subject is harboring a nucleic acid selected from the group consisting of SEQ ID NO:

2, SEQ ID NO;

3, SEQ ID NO;

4, wherein the method comprises the steps of

(a) obtaining a sample from said subject

(b) testing the sample for the presence of said nucleotide sequence; and

(c) detecting the presence of said sequences wherein the detection indicates the presence of viral induced obesity in said subject.

2. A method for testing a subject for viral obesity comprising the steps of

obtaining a sample of nucleic acids from the subject;

testing the sample of nucleic acids for the presence of nucleotide sequences selected from the group consisting of SEQ ID NO;

2, SEQ ID NO;

3 and SEQ ID NO;

4; and

detecting the presence of said sequences wherein the detection indicates the presence of viral obesity in said subject.

3. An isolated DNA molecule having a sequence selected from the group consisting of SEQ ID NO:

2, SEQ ID NO;

3 and SEQ ID NO;

4.

4. A peptide encoded by a DNA sequence of claim 3.