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Process to create biomolecule and/or cellular arrays on metal surfaces and product produced thereby

  • US 6,127,129 A
  • Filed: 08/05/1999
  • Issued: 10/03/2000
  • Est. Priority Date: 05/04/1999
  • Status: Expired due to Fees
First Claim
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1. A method of making a biomolecule or cellular array on a metal substrate comprising:

  • (a) depositing an ω

    -modified alkanethiol monolayer on a metal substrate;

    (b) reacting hydrophobic protecting groups with the monolayer;

    (c) patterning the monolayer to create an array of exposed metal substrate areas;

    (d) depositing ω

    -modified alkanethiol in the areas of exposed metal substrate, thereby yielding an array of discrete, unprotected ω

    -modified alkanethiol spots;

    (e) attaching biomolecules or cells to the discrete, unprotected ω

    -modified alkanethiol spots, thereby yielding an array of discrete spots having biomolecules or cells immobilized thereon;

    (f) removing the protecting groups of step (b); and

    then(g) making the monolayer resistant to non-specific protein binding wherein the method results in a biomolecule or cellular array.

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