Assay for quantitative measurement of analytes in biological samples
First Claim
1. A method for determining concentration of an analyte in a biological sample comprising the steps of:
- (a) combining the biological sample, at least one oxidizing enzyme for the analyte of interest, nicotinamide adenine dinucleotide, and a chemiluminescent label to form a reaction mixture;
(b) allowing the analyte to undergo an oxidation-reduction reaction and nicotinamide adenine dinucleotide to be converted to the reduced form of nicotinamide adenine dinucleotide and further allowing the chemiluminescent label to react with the reduced form of nicotinamide adenine dinucleotide; and
(c) determining the concentration of the analyte of interest in the biological sample by correlating the quantity of light emitted with the concentration of the reduced form of nicotinamide adenine dinucleotide.
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Abstract
A method for determining concentration of an analyte in a biological sample comprising the steps of:
(a) combining the biological sample, at least one oxidizing enzyme for the analyte of interest, nicotinamide adenine dinucleotide (hereinafter NAD+), and a chemiluminescent label to form a reaction mixture;
(b) allowing the analyte to undergo an oxidation-reduction reaction and NAD+ to be converted to the reduced form of nicotinamide adenine dinucleotide (hereinafter NADH) and further allowing the chemiluminescent label to react with NADH; and
(c) determining the concentration of the analyte of interest in the biological sample by correlating the quantity of light emitted with the concentration of NADH.
15 Citations
28 Claims
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1. A method for determining concentration of an analyte in a biological sample comprising the steps of:
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(a) combining the biological sample, at least one oxidizing enzyme for the analyte of interest, nicotinamide adenine dinucleotide, and a chemiluminescent label to form a reaction mixture; (b) allowing the analyte to undergo an oxidation-reduction reaction and nicotinamide adenine dinucleotide to be converted to the reduced form of nicotinamide adenine dinucleotide and further allowing the chemiluminescent label to react with the reduced form of nicotinamide adenine dinucleotide; and (c) determining the concentration of the analyte of interest in the biological sample by correlating the quantity of light emitted with the concentration of the reduced form of nicotinamide adenine dinucleotide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for determining concentration of an analyte in a biological sample comprising the steps of:
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(a) combining the biological sample, a solid phase, at least one oxidizing enzyme for the analyte of interest, nicotinamide adenine dinucleotide, and a chemiluminescent label to form a reaction mixture; (b) allowing the analyte to undergo an oxidation-reduction reaction and nicotinamide adenine dinucleotide to be converted to the reduced form of nicotinamide adenine dinucleotide and further allowing the chemiluminescent label to react with the reduced form of nicotinamide adenine dinucleotide; (c) separating the chemiluminescent label from the solid phase; and (d) determining the concentration of the analyte in the biological sample by correlating quantity of light emitted with the concentration of the reduced form of nicotinamide adenine dinucleotide. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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Specification