Methods for direct synthesis of compounds having complementary structure to a desired molecular entity and use thereof
First Claim
1. A method for producing a compound suitable for in vitro or in vivo usage as a diagnostic, therapeutic, purification, separation, or prophylactic agent that possesses a complementary structure to a specific site on a target molecular entity or portion thereof by site-specific molecular imprinting comprising the following steps:
- (i) selecting a particular molecular entity to which a compound having a complementary structure is to be produced;
(ii) contacting such molecular entity with one or more complementary monomers under conditions wherein such monomers associate around one or more residues of such molecular entity;
(iii) effecting polymerization of such associated monomers optionally in the presence of at least one crosslinking agent to produce a polymeric coating on the surface of said molecular entity and which possesses a complementary structure to said biological entity or a portion thereof;
(iv) removing said molecule under conditions that result in a polymeric compound that possesses a complementary structure to said molecular entity or portion thereof; and
(v) optionally effecting one or more cleavage and/or dissociation steps to produce compounds that are suitable for in vivo or in vitro usage as a diagnostic, therapeutic, purification, separation and/or prophylactic agent.
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Accused Products
Abstract
Compounds which possess a complementary structure to a desired molecule, such as a biomolecule, in particular polymeric or oligomeric compounds, which are useful as in vivo or in vitro diagnostic and therapeutic agents are provided. Also, various methods for producing such compounds are provided. These polymeric or oligomeric compounds are useful in particular as antimicrobial agents, receptor, hormone or enzyme agonists and antagonists.
186 Citations
38 Claims
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1. A method for producing a compound suitable for in vitro or in vivo usage as a diagnostic, therapeutic, purification, separation, or prophylactic agent that possesses a complementary structure to a specific site on a target molecular entity or portion thereof by site-specific molecular imprinting comprising the following steps:
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(i) selecting a particular molecular entity to which a compound having a complementary structure is to be produced; (ii) contacting such molecular entity with one or more complementary monomers under conditions wherein such monomers associate around one or more residues of such molecular entity; (iii) effecting polymerization of such associated monomers optionally in the presence of at least one crosslinking agent to produce a polymeric coating on the surface of said molecular entity and which possesses a complementary structure to said biological entity or a portion thereof; (iv) removing said molecule under conditions that result in a polymeric compound that possesses a complementary structure to said molecular entity or portion thereof; and (v) optionally effecting one or more cleavage and/or dissociation steps to produce compounds that are suitable for in vivo or in vitro usage as a diagnostic, therapeutic, purification, separation and/or prophylactic agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 27, 31, 32, 33, 34, 37)
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14. A method for producing a polymeric compound that exhibits complementary structure to a specific site on a cell or virus by site specific molecular imprinting comprising the following steps:
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(i) optionally immobilizing said cell or virus to a support; (ii) coating said optionally immobilized cell or virus with a polymer that is crosslinkable under specific conditions; (iii) selectively crosslinking the portion of the coating that is proximate to the cell or virus; and (iv) removing the resultant molecular coating from the cell or virus. - View Dependent Claims (15, 16, 17, 18, 19, 20, 28)
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21. A method for producing a compound that has a complementary structure to the active or binding site of a molecular entity by site-specific molecular imprinting comprising the following steps:
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(i) providing a support which is coated with a first monomer layer coating; (ii) applying to said first layer a second layer which comprises at least one crosslinkable monomer which is able to move freely in said second layer; (iii) exposing said second layer to a molecular entity containing at least one active or binding site and allowing for said crosslinkable monomer to associate around said at least one active site; (iv) providing a crosslinking agent and effecting crosslinking to produce a crosslinked compound that possesses a complementary structure to said at least one active or binding site; and (v) recovering said crosslinked compound that possesses a complementary structure to said at least one active or binding site or sites. - View Dependent Claims (22, 23, 29)
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24. A method for producing a polymeric compound that has a complementary structure to a specific site on a molecular entity by site-specific molecular imprinting which method comprises the following steps:
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(i) providing a preformed functionalized polymer that is linear or partially crosslinked and contacting same with a molecular entity such that specific functional groups on the polymer interact covalently or non-covalently with specific residues on the molecular entity; (ii) allowing for such non-covalent or covalent interactions between the functional groups on the polymer and the molecular entity to equilibrate; (iii) subjecting the resultant equilibrated covalent or non-covalent complex between the polymer and the molecular entity to at least one of the following steps; (1) chemically treating the functional groups on the polymer that interact least strongly with the molecular entity by site-selective chemical modification; (2) chemically treating the functional groups on the polymer that interact most strongly with the molecular entity by site-selective chemical modification; and
/or(3) crosslinking the polymer; (iv) separating the molecular entity from the resultant polymer. - View Dependent Claims (25, 26, 30, 38)
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35. A method for producing a compound suitable for in vitro or in vivo usage as a therapeutic agent that possesses a complementary structure to a specific site on a target molecule or portion thereof by site-specific molecular imprinting comprising the following steps:
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(i) selecting a particular molecule to which a compound having a complementary structure is to be produced; (ii) contacting such molecule with one or more complementary monomers under conditions wherein such monomers associate around one or more residues of such molecule; (iii) effecting polymerization of such associated monomers optionally in the presence of at least one crosslinking agent to produce a polymeric coating which possesses a complementary structure to said specific site of said molecule or a portion thereof; (iv) removing said molecule to produce a compound that possesses a complementary structure to said specific site of said molecule or portion thereof; and (v) optionally effecting one or more cleavage and/or dissociation steps to produce a compound that is suitable for in vivo usage as a therapeutic agent. - View Dependent Claims (36)
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Specification