Confocal spectroscopy system and method

• US 6,128,077 A
• Filed: 11/17/1998
• Issued: 10/03/2000
• Est. Priority Date: 11/17/1997
• Status: Expired due to Term

• Current Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Original Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Inventors: Jovin, Thomas M., Verveer, Peter, Hanley, Quentin
• Primary Examiner(s): Font, Frank G.
• Assistant Examiner(s): LAUCHMAN, LAYLA G
• Application Number:
  US09/193,692
• Time in Patent Office: 686 Days
• Field of Search: 356/310, 356/330, 356/326, 356/328, 359/310, 359/341
• US Class Current: 356/310
• CPC Class Codes: G01J 2003/2866 Markers; Calibrating of scan
• CPC Class Codes: G01J 3/021 using plane or convex mirrors, parallel phase plates, or particular reflectors
• CPC Class Codes: G01J 3/10 Arrangements of light sources specially adapted for spectrometry or colorimetry
• CPC Class Codes: G01J 3/2823 Imaging spectrometer
• CPC Class Codes: G01J 3/457 Correlation spectrometry, e.g. of the intensity G01J3/453 takes precedence
• CPC Class Codes: G02B 21/004 fixed arrays, e.g. switchable aperture arrays
• CPC Class Codes: G02B 21/0076 arrangements using fluorescence or luminescence

Abstract

A confocal spectral imaging system comprises a light source, a light modulator forming an illumination aperture and directing an illumination pattern to conjugate object locations, and analyzing means with a detection aperture, dispersive elements and a detector, wherein the illumination and detection apertures are in conjugate optical planes, and the light modulator consist of an array of light modulator elements, a group of which being arranged according to the illumination pattern and forming the illumination aperture, and are controlled such that the illumination pattern is directed to time-dependent changing conjugate locations of the object. A programmable light source comprises a white light source, dispersion means and a spatial light modulator with an array of individually time-dependent controllable modulator elements being illuminated with the dispersed light and providing a position selective transmittivity or reflectivity, so that a light with a predetermined wavelength distribution passes the light modulator.

Claims

1. A confocal spectral imaging system comprising:

light source means;

light modulator means being arranged in a light path between said light source means and an object under investigation, said light modulator means comprising a two-dimensional array of light modulator elements, a group of which being arranged according to a predetermined illumination pattern to be formed in conjugate locations of said object, said light modulator elements forming an illumination aperture, wherein said light modulator means is controllable such that the illumination pattern is directed to time-dependent changing conjugate locations of the object, and

analyzing means with spectrally dispersive elements and a detector camera, said analyzing means capable of collecting from said object spectrally resolved light with multiple wavelengths in a dispersed state, wherein a detection aperture is arranged in a light path between said object and said spectrally dispersive elements, and said illumination and detection apertures are placed in conjugate optical planes.

2. The system according to claim 1, wherein the light modulator elements are individually controllable such that the reflective, transmissive or diffractive properties thereof are changed.

3. The system according to claim 1, wherein the light modulator means are adapted to scan at least a part of the object according to a predetermined illumination pattern sequence.

4. The system according to claim 3, wherein the pattern sequence is selected from the group consisting of:

a systematically shifting line or lines either spaced regularly, according to a pseudo-random sequence of finite length, according to a S-matrix type Hadamard sequence or randomly;

regular dot lattices such as triangles, squares, rectangles, hexagons;

random patterns or pseudo-random patterns of finite length based on so-called Walsch, Sylvester, Hadamard or Golay sequences;

square or rectangular grids formed from intersecting line patterns;

plane filling patterns being adapted to turn on the scanning laser microscopy elements an integral number of times when sequentially produced on the scanning laser microscopy;

or a combination of the above pattern sequences.

5. The system according to claim 1, wherein the detection aperture is formed by the pattern shaped group of modulator elements so that the conjugate optical planes for illumination and detection coincide.

6. The system according to claim 1, wherein the entrance plane of the spectrally dispersive elements and the two-dimensional detector camera are arranged in conjugate optical planes.

7. The system according to claim 1, wherein the entrance plane of the spectrally dispersive elements is defined by the two-dimensional array of light modulator elements.

8. The system according to claim 1, being a part of a confocal programmable array microscope and further comprising full field detection means with a two-dimensional camera for collecting a conjugate or a non-conjugate microscopic image of the object.

9. The system according to claim 1, wherein the light source means contain a white light lamp and a fixed or tunable wavelength filter which comprises a spectrally dispersive element and another two-dimensional array of individually time-dependent controllable light modulator elements being illuminated with dispersed light from the spectrally dispersive element and providing a position selective transmittivity or reflectivity for producing light with a predetermined wavelength distribution.

10. The system according to claim 1, wherein the detection light is fluorescence, phosphorescence, reflected light or Raman scattered light emitted from the object.

18. The system according to claim 1, wherein said light source means comprises a programmable light source comprising a white or broad band light source, dispersion means for wavelength-selection of light from the light source, spatial light modulator means with a two-dimensional array of individually time-dependent controllable light modulator elements being illuminated with the dispersed light and providing a position selective transmittivity or reflectivity, so that a light output with a predetermined wavelength distribution passes the spatial light modulator means.

19. The system according to claim 18, further comprising a device for recombining the dispersed wavelengths being an integrating sphere, a lens system adapted for illumination in parallel mode, a matching set of dispersion optics, fiber optics, liquid light conduits, or scattering surfaces or materials.

20. The system according to claim 18, wherein the modulator elements are controllable with a transmission or reflection time dependent pattern corresponding to rows or columns of an S-type Hadamard coding matrix or with a predetermined time sweep.

22. The system according to claim 1 wherein said light source means comprises a programmable light source comprising a white or broad band light source, dispersion means for wavelength-selection of light from the light source, spacial light modulator means with a two-dimensional array of individually time-dependent controllable light modulator elements being illuminated with the dispersed light and providing a position selective transmittivity or reflectivity, so that a light output with a predetermined wavelength distribution passes the spatial light modulator means.

23. The system according to claim 22 wherein said programmable light source further comprises a device for recombining the disbursed wavelengths being an integrating sphere, a lens system adapted for illumination in parallel mode (Kohler-type illumination), a matching set of dispersion optics, fiber optics, liquid light conduits, or scattering surfaces or materials.

24. The system according to claim 22 wherein the modulator elements are controllable with a transmission or reflection time dependent pattern corresponding to rows or columns of an S-type Hadamard coding matrix or with a predetermined time sweep.

11. A confocal spectral imaging method, comprising the steps of:

focusing illumination light from a light source means via light modulator means forming an illumination aperture and shaping a predetermined illumination pattern, to conjugate locations of an object under investigation, wherein said light modulator means comprises a two-dimensional array of light modulating elements which are controlled in a time-dependent fashion such that light modulating elements shaped according to the illumination aperture form an illumination pattern sequence, and

collecting spectrally resolved light with multiple wavelengths in a dispersed state emitted from said object with analyzing means with a detection aperture, spectrally dispersive elements and a detector camera, wherein the illumination and detection apertures are placed in conjugate optical planes.

12. The method according to claim 11, wherein the light modulating elements are controlled such that at least a part of the object is scanned according to the predetermined pattern sequence, wherein the illumination patterns are selected from a group consisting of:

a systematically shifting line or lines either spaced regularly, according to a pseudo-random sequence of finite length, according to a S-matrix type Hadamard sequence or randomly;

regular dot lattices such as triangles, squares, rectangles, hexagons;

random patterns or pseudo-random patterns of finite length based on so-called Walsch, Sylvester, Hadamard or Golay sequences;

square or rectangular grids formed from intersecting line patterns;

plane filling patterns being adapted to turn on the scanning laser microscopy elements an integral number of times when sequentially produced on the scanning laser microscopy;

or a combination of the above pattern sequences.

13. The method according to claim 11, wherein simultaneously spectrally resolved conjugate and/or non-conjugate images of the object are collected with full field detector means.

14. The method according to claim 11, wherein the step of focusing light includes a wavelength selection.

15. The method according to claim 14, comprising the further step of:

deconvoluting the object with a deconvolution algorithm so as to reconstruct the spectrally resolved conjugate and non-conjugate images.

16. The method according to claim 11, comprising the further step of:

simultaneously initiating and monitoring photochemical reactions on a substrate, wherein the initiating includes the irradiation of predetermined substances with a suitable wavelength and the monitoring includes fluorescence measurements with the substances or the reaction products.

17. The method according to claim 11, wherein the method is used in process selected from the group consisting of:

investigations in the cell and tissue biology,

analytical/biotechnological procedures,

in-situ hybridization in genetic analysis,

formation of an optical mask for position selective photochemical reactions,

position selective spectroscopic measurements including excitation and emission spectra of one or more intrinsic or external fluorphores, lifetime measurements polarization measurements and/or absorptions measurements in a reflection mode,

readout of biochemical arrays on chips,

large scale surface inspections in the semiconductor industry, and

optical recording and read-out.

21. The method according to claim 13 wherein the step of focusing light includes a wavelength selection.

25. A spectral imaging system comprising:

a light source;

a light modulation system disposed in an illumination path between said light source and an object under investigation; and

,

a spectral analyzer disposed in a detection path, said detection path including said object and being different from said illumination path, wherein said system allows simultaneous detection of multiple wavelengths.

26. The spectral imaging system according to claim 25, wherein said light modulation system comprises a two-dimensional array of light modulator elements, said light modulator elements forming an illumination aperture, a group of said light modulator elements being arranged according to a predetermined illumination pattern.

27. The spectral imaging system according to claim 25, wherein said analyzer comprises a set of spectrally dispersive elements and a detector camera, said analyzer collecting from said object spectrally resolved light with multiple wavelengths in a dispersed state, and wherein a detection aperture is arranged in a light path between said object and said spectrally dispersive elements.

28. The spectral imaging system according to claim 25, wherein said analyzer provides spectrally resolved detection.

29. A method for spectral imaging, comprising the steps of:

focusing illumination light from a light source on a subject via a light modulation system, said light modulation system forming an illumination aperture and shaping a predetermined illumination pattern, the light modulation system comprising a two-dimensional array of light modulation elements; and

,

collecting spectrally resolved light with multiple wavelengths in a dispersed state emitted from said object with a spectral analyzer.