Use of CYB medium for the transportation and storage of sperm

• US 6,130,034 A
• Filed: 05/08/1998
• Issued: 10/10/2000
• Est. Priority Date: 11/09/1995
• Status: Expired due to Term

• Current Assignee: Medical Research Council (Government of United Kingdom), Merck Serono SA (Merck & Co., Inc.)
• Original Assignee: Medical Research Council (Government of United Kingdom), Applied Research Systems ARS Holding NV (Merck & Co., Inc.)
• Inventors: Aitken, Robert John
• Primary Examiner(s): Saucier, Sandra E.
• Application Number:
  US09/074,465
• Time in Patent Office: 886 Days
• Field of Search: 435/2, 600/33, 600/35
• US Class Current: 435/2
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0226 Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients

Abstract

The use of CYB medium for the storage and/or transportation of semen at ambient temperature.

Claims

1. A method for the storage and/or transportation of semen at ambient temperature which comprises the step of bringing the semen into contact with a medium comprising CYB medium, wherein said ambient temperature does not reach a level required for cryopreservation of sperm, and wherein the time period for said storage and/or transportation exceeds 1 hour.

2. The method as claimed in claim 1, wherein the semen is diluted 1:

1 with the medium.

3. The method as claimed in claim 1, wherein the semen is human semen.

4. The method as claimed in claim 1, wherein the medium in addition to the CYB medium further comprises an antioxidant in sufficient quantity to prevent loss of motility.

5. The method as claimed in claim 4, wherein the antioxidant is α

-tocopherol, catalase, glutathione or mannitol.

6. The method as claimed in claim 5, wherein the antioxidant is α

-tocopherol and is present at a concentration of 1 mM.