Surfaces for biological reactions, process for preparing them and process for their use

• US 6,130,044 A
• Filed: 05/26/1998
• Issued: 10/10/2000
• Est. Priority Date: 02/11/1994
• Status: Expired due to Term

• Current Assignee: INSTITUT PASTEUR AND CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
• Original Assignee: INSTITUT PASTEUR AND CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
• Inventors: Bensimon, Aaron, Bensimon, David, Heslot, Fran.cedilla.ois
• Primary Examiner(s): Stucker, Jeffrey
• Assistant Examiner(s): BASKAR, PADMAVATHI
• Application Number:
  US09/084,004
• Time in Patent Office: 868 Days
• Field of Search: 436/63, 436/96, 436/532, 436/501, 435/5-6, 435/970, 435/969, 524/511, 935/77, 935/78, 422/104
• US Class Current: 435/6.12
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00628 Ionic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/682 Signal amplification
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 2565/518 characterised by the immobilisation of the nucleic acid sample or target
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 33/6803 : General methods of protein analysis not limited to specific proteins or families of proteins
• CPC Class Codes: Y10S 435/969 : Multiple layering of reactants
• CPC Class Codes: Y10S 435/97 : Test strip or test slide
• CPC Class Codes: Y10S 530/81 : Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
• CPC Class Codes: Y10S 530/811 : Peptides or proteins is immobilized on, or in, an inorganic carrier
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/145555 : Hetero-N

Abstract

A highly specific surface for biological reactions, which includes a support with at least one essentially compact layer of an organic compound. On the outside of the organic compound layer is an exposed group containing an ethylenic double bond, which has affinity for a molecule with biological activity under certain reaction conditions. The other elements of the organic compound layer are essentially inaccessible to the molecule with biological activity under the reaction conditions.

Claims

1. A surface for biological reactions, comprising a support having at least one essentially compact layer of an organic compound having, outside the layer, an exposed group containing an ethylenic double bond having affinity for a molecule with biological activity, wherein the remainder of the layer is essentially inaccessible to the molecule with biological activity and further wherein one end of the molecule with biological activity is attached to the surface.

2. The surface according to claim 1, wherein the molecule with biological activity is a protein, a nucleic acid, a lipid, or a polysaccharide.

3. The surface according to claim 1, wherein the molecule with biological activity is a protein, DNA, or RNA.

4. The surface according to claim 1, wherein the molecule with biological activity is an antibody, an antigen, a ligand, or a ligand receptor.

5. The surface according to claim 1, which is coated with a molecule with biological activity, wherein said surface is highly specific for a biological reaction.

6. The surface according to claim 5, wherein the molecule with biological activity is a protein, a nucleic acid, a lipid, or a polysaccharide.

7. A process for detecting a DNA sequence or a protein in a sample, comprising the steps of:

(A) contacting the sample with a surface according to claim 3,

(B) binding the sample DNA sequence or protein with the surface to form a DNA/DNA, DNA/RNA, or protein/protein hybrid,

(C) stretching said hybrid by physicochemical means in order to orientate said hybrid, and

(D) detecting said stretched hybrid.

8. The process according to claim 7, wherein the hybrid is stretched by passage of a meniscus in the direction of the stretching.

9. The process according to claim 8, wherein the meniscus is an air/water meniscus.

10. The process according to claim 7, wherein the stretching is carried out by an electric field acting on the molecules or a magnetic field acting on a magnetizable or magnetic particle.

11. The process according to claim 7, wherein the stretching is carried out mechanically.

12. The process according to claim 7, further comprising the step of subjecting said hybrid to a stress so as to destroy the mismatches before detection.

13. The process according to claim 12, wherein the hybrid is stretched by passage of a meniscus in the direction of the stretching.

14. The process according to claim 13, wherein the meniscus is an air/water meniscus.

15. The process according to claim 12, wherein the stretching is carried out by an electric field acting on the molecules or a magnetic field acting on a magnetizable or magnetic particle.

16. The process according to claim 12, wherein the stretching is carried out mechanically.