Miniature reaction chamber and devices incorporating same
First Claim
Patent Images
1. A miniature reaction system comprising:
- a body having a cavity disposed therein;
a heater deposited within said cavity, said heater being electrically connected to a power source;
a temperature sensor disposed on a surface of said body within said cavity;
an appropriately programmed computer for monitoring said temperature and controlling said power source; and
a microcapillary gel electrophoresis device comprising a first gel filled microcapillary extending from the cavity, and a second gel filled microcapillary intersecting across the first gel filled microcapillary, the second gel filled microcapillary being in fluid communication with the first gel filled microcapillary, said second gel filled microcapillary comprising an electrophoretic channel.
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Abstract
The present invention generally relates to miniaturized devices for carrying out and controlling chemical reactions and analyses. In particular, the present invention provides devices which have miniature temperature controlled reaction chambers for carrying out a variety of synthetic and diagnostic applications, such as PCR amplification, nucleic acid hybridization, chemical labeling, nucleic acid fragmentation and the like.
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Citations
38 Claims
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1. A miniature reaction system comprising:
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a body having a cavity disposed therein; a heater deposited within said cavity, said heater being electrically connected to a power source; a temperature sensor disposed on a surface of said body within said cavity; an appropriately programmed computer for monitoring said temperature and controlling said power source; and a microcapillary gel electrophoresis device comprising a first gel filled microcapillary extending from the cavity, and a second gel filled microcapillary intersecting across the first gel filled microcapillary, the second gel filled microcapillary being in fluid communication with the first gel filled microcapillary, said second gel filled microcapillary comprising an electrophoretic channel. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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2. A miniature reaction system comprising:
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a body having a cavity disposed therein; a heater deposited within said cavity, said heater being electrically connected to a power source; a temperature sensor disposed on a surface of said body within said cavity; an appropriately programmed computer for monitoring said temperature and controlling said power source; a gel filled microcapillary extending from the cavity; and a reaction chamber in fluid communication with the gel filled microcapillary, the reaction chamber having a nucleic acid array disposed therein, said nucleic acid array including a substrate having a plurality of positionally distinct nucleic acid probes coupled to a surface of said substrate.
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- 12. The reaction system of claim 1or 2, wherein said temperature sensor comprises a thermocouple separate from the heater and having a sensing junction positioned adjacent said cavity, and a reference junction positioned outside of said cavity, said thermocouple being electrically connected to a voltage measuring device.
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28. A miniature reaction system comprising:
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a body having a cavity disposed therein wherein said body comprises at least first and second planar members, said first planar member having a first surface and a well disposed in said first surface, said second planar member having a second substantially planar surface, said second surface being mated to said first surface whereby said well forms said cavity; a resistive heater deposited within said cavity, said heater being electrically connected to a power source for applying a voltage across said heater; a temperature sensor disposed on a surface of said body within said cavity, for determining a temperature within said cavity, whereby a temperature at said temperature sensor is substantially the same as a temperature within said cavity; an appropriately programmed computer for monitoring said temperature and controlling said power source to selectively apply said current across said heater; and wherein said temperature sensor is deposited on said second surface, and wherein when said second surface is mated with said first surface.
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29. A miniature reaction system comprising:
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a body having a cavity disposed therein; a resistive heater deposited within said cavity, said heater being electrically connected to a power source for applying a voltage across said heater; a temperature sensor disposed on a surface of said body within said cavity, for determining a temperature within said cavity, whereby a temperature at said temperature sensor is substantially the same as a temperature within said cavity; an appropriately programmed computer for monitoring said temperature and controlling said power source to selectively apply said current across said heater; and wherein said power source delivers an AC voltage across said resistive heater.
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30. A method of cycling a temperature of a reaction mixture for amplification of an oligonucleotide, comprising:
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depositing said reaction mixture into a microfabricated reaction chamber, said microfabricated reaction chamber having a microfabricated thin film heating element disposed therein; applying an AC voltage to said microfabricated thin film heating element; cycling said applying step to raise and lower said a temperature of said element, and thereby raise and lower said temperature of said reaction mixture. - View Dependent Claims (31, 32, 33, 34, 35)
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36. A method of cycling a temperature of a reaction mixture for amplification of an oligonucleotide and separating a reaction product of the reaction mixture, comprising:
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depositing said reaction mixture into a microfabricated reaction chamber, said microfabricated reaction chamber having a microfabricated thin film heating element disposed therein; applying a voltage to said microfabricated thin film heating element; cycling said applying step to raise and lower said a temperature of said element, and thereby raise and lower said temperature of said reaction mixture; electrophoretically loading the reaction product of the reaction mixture through a first gel filled microcapillary and across a second gel filled microcapillary, wherein the first and second gel filled microcapillaries intersect one another; and electrophoretically separating in the second gel filled microcapillary a plug of the reaction product deposited in the intersection of the first and second gel filled microcapillaries.
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37. A method of cycling a temperature of a reaction mixture for amplification of an oligonucleotide and separating a reaction product of the reaction mixture, comprising:
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depositing said reaction mixture into a microfabricated reaction chamber, said microfabricated reaction chamber having a microfabricated thin film heating element disposed therein; applying a voltage to said microfabricated thin film heating element; cycling said applying step to raise and lower said a temperature of said element, and thereby raise and lower said temperature of said reaction mixture; electrophoretically loading the reaction product of the reaction mixture through a first gel filled microcapillary and into a second gel filled microcapillary, wherein the first and second gel filled microcapillaries intersect one another; and electrophoretically separating in the second gel filled microcapillary a stack of the reaction product deposited in the second gel filled microcapillary.
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38. A method of cycling a temperature of a reaction mixture for amplification of an oligonucleotide and injecting a reaction product of the reaction mixture into a second reaction chamber, comprising:
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depositing said reaction mixture into a microfabricated reaction chamber, said microfabricated reaction chamber having a microfabricated thin film heating element disposed therein; applying a voltage to said microfabricated thin film heating element; cycling said applying step to raise and lower said a temperature of said element, and thereby raise and lower said temperature of said reaction mixture; and electrophoretically transporting a reaction product of the reaction mixture through a gel filled microcapillary and into the second reaction chamber, wherein the second reaction chamber has a nucleic acid array disposed therein.
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Specification